Characterization of Silver Carbonate Nanoparticles Biosynthesized Using Marine <i>Actinobacteria</i> and Exploring of Their Antimicrobial and Antibiofilm Activity
Omar Messaoudi,
Ibrahim Benamar,
Ahmed Azizi,
Salim Albukhaty,
Yasmina Khane,
Ghassan M. Sulaiman,
Mounir M. Salem-Bekhit,
Kaouthar Hamdi,
Sirine Ghoummid,
Abdelhalim Zoukel,
Ilhem Messahli,
Yacine Kerchich,
Farouk Benaceur,
Mohamed M. Salem,
Mourad Bendahou
Affiliations
Omar Messaoudi
Department of Biology, Faculty of Science, University of Amar Telidji, Laghouat 03000, Algeria
Ibrahim Benamar
Department of Biology, Faculty of Science, University of Amar Telidji, Laghouat 03000, Algeria
Ahmed Azizi
Department of The Common Trunk Sciences and Technology, Faculty of Technology, University of Amar Telidji, Highway Ghardaia, P.O. Box G37 (M’kam), Laghouat 03000, Algeria
Salim Albukhaty
Department of Chemistry, College of Science, University of Misan, Maysan 62001, Iraq
Yasmina Khane
Faculty of Science and Technology, University of Ghardaia, BP455, Ghardaia 47000, Algeria
Ghassan M. Sulaiman
Division of Biotechnology, Department of Applied Sciences, University of Technology, Baghdad 10066, Iraq
Mounir M. Salem-Bekhit
Department of Pharmaceutics, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia
Kaouthar Hamdi
Department of Biology, Faculty of Science, University of Amar Telidji, Laghouat 03000, Algeria
Sirine Ghoummid
Department of Biology, Faculty of Science, University of Amar Telidji, Laghouat 03000, Algeria
Abdelhalim Zoukel
Laboratory Physico-Chemistry of Materials, Laghouat University, Laghouat 03000, Algeria
Ilhem Messahli
Department of Biology, Faculty of Science, University of Amar Telidji, Laghouat 03000, Algeria
Yacine Kerchich
École Nationale Polytechnique (ENP), Laboratory of Environmental Science and Technology, El Harrach 16200, Algeria
Farouk Benaceur
Department of Biology, Faculty of Science, University of Amar Telidji, Laghouat 03000, Algeria
Mohamed M. Salem
College of Medicine, Huazhong University of Science and Technology, Wuhan 430030, China
Mourad Bendahou
Laboratory of Applied Microbiology in Food and Environment, Abou Bekr Belkaïd University, Tlemcen 13000, Algeria
Bacterial resistance to different antimicrobial agents is growing with alarming speed, especially when bacterial cells are living in biofilm. Hybrid nanoparticles, synthesized through the green method, hold promise as a potential solution to this challenge. In this study, 66 actinomycete strains were isolated from three distinct marine sources: marine sediment, the algae Codium bursa, and the marine sponge Chondrosia reniformis. From the entirety of the isolated strains, one strain, S26, identified as Saccharopolyspora erythrea, was selected based on its taxonomic position and significant antimicrobial activity. Using the biomass of the selected marine Actinobacteria, the green synthesis of eco-friendly silver carbonate nanoparticles (BioAg2CO3NPs) is reported for the first time in this pioneering study. The BioAg2CO3NPs were characterized using different spectroscopic and microscopic analyses; the synthesized BioAg2CO3NPs primarily exhibit a triangular shape, with an approximate size of 100 nm. Biological activity evaluation indicated that the BioAg2CO3NPs exhibited good antimicrobial activity against all tested microorganisms and were able to remove 58% of the biofilm formed by the Klebsiella pneumoniae kp6 strain.
