Directed evolution of phosphite dehydrogenase to cycle noncanonical redox cofactors via universal growth selection platform

Linyue Zhang; Edward King; William B. Black; Christian M. Heckmann; Allison Wolder; Youtian Cui; Francis Nicklen; Justin B. Siegel; Ray Luo; Caroline E. Paul; Han Li

doi:10.1038/s41467-022-32727-w

Nature Communications (Aug 2022)

Directed evolution of phosphite dehydrogenase to cycle noncanonical redox cofactors via universal growth selection platform

Linyue Zhang,
Edward King,
William B. Black,
Christian M. Heckmann,
Allison Wolder,
Youtian Cui,
Francis Nicklen,
Justin B. Siegel,
Ray Luo,
Caroline E. Paul,
Han Li

Affiliations

Linyue Zhang: Department of Chemical and Biomolecular Engineering, University of California Irvine
Edward King: Department of Molecular Biology and Biochemistry, University of California Irvine
William B. Black: Department of Chemical and Biomolecular Engineering, University of California Irvine
Christian M. Heckmann: Biocatalysis, Department of Biotechnology, Delft University of Technology
Allison Wolder: Biocatalysis, Department of Biotechnology, Delft University of Technology
Youtian Cui: Department of Chemistry, University of California, Davis, One Shields Avenue
Francis Nicklen: Department of Chemical and Biomolecular Engineering, University of California Irvine
Justin B. Siegel: Department of Chemistry, University of California, Davis, One Shields Avenue
Ray Luo: Department of Chemical and Biomolecular Engineering, University of California Irvine
Caroline E. Paul: Biocatalysis, Department of Biotechnology, Delft University of Technology
Han Li: Department of Chemical and Biomolecular Engineering, University of California Irvine

DOI: https://doi.org/10.1038/s41467-022-32727-w
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 12

Abstract

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Engineering enzymes to utilize the noncanonical redox cofactors such as nicotinamide mononucleotide (NMN + ) is challenging. Here, the authors report a growth-based selection platform for NMN + -reducing enzyme engineering and show its application in developing a phosphite dehydrogenase with improved catalytic efficiency.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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