Identification of 11 microsatellites markers in Bulgarian Rhodope cattle breed

Abstract

The purpose of present work was to study the genetic variation in 135 animals from Bulgarian Rhodope cattle breed raised in the region of Smolyan, Bulgaria. For the purpose of the experiment was used a panel of 11 microsatellite markers. A total of 113 alleles were identified and 5 of them were population-specific. The allele number per locus varied from 7 (for SPS115) to 13 (for TGLA 227, TGLA 53 and INRA023 loci) with a mean number of 10.27. Allele frequency varied in different microsatellite loci. In locus TGLA227 the most frequent allele was with length 97 bp (0.59), and the rarest allele was with length 103 bp (0.025). The highest number of heterozygotes (125) was observed in locus INRA023 (n = 135). The lowest number of heterozygotes (42) was detected in locus TGLA 122 (n = 135). Microsatellite markers used in the current experiment showed PIC from 0.73 (loci SPS115 and BM2113) to 0.86 (loci INRA023 and TGLA227) with mean value of 0.78. Observed heterozygosity (Ho) varied from 0.828 (locus ETH3) to 0.959 (loci INRA23 and TGLA227). Expected heterozygosity (genetic diversity - He) varied from 0.558 (loci ETH10 and BM1824) to 0.849 (locus INRA23). The mean heterozygosity for all investigated loci was Ho = 0.913 and He = 0.734. Estimated values (PIC, Ho, He и MNA) showed that all studied markers were polymorphic. All tested loci were with high polymorphic information content (>0.5) and Ho > 0.6.

Keywords

• bulgarian rhodope cattle
• genetic diversity
• ssr markers
• polymorphism