Molecular imprinted polymer based sensor for recognition and determination of profenofos organophosphorous insecticide

Abstract

A selective and sensitive electrochemical sensor for recognition of profenofos (PFF) was fabricated based on the drop coating of synthesized molecularly imprinted polymers (MIP) and graphene oxide (GO) on the surface of glassy carbon (GCE). The imprinted layer was systematically characterized by scanning electron microscopy (SEM) and Fourier-transform infrared spectroscopy (FTIR). Electro chemical studies were conducted with cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) through a “gate-controlled” effect using [Fe (CN)6] 3-/4- as a probe. Under optimal condition, the modified GCE exhibited linear calibration curve for PFF over a concentration range from 5 × 10−8 to 35 × 10−4 M with a detection limit of 5 nM. The proposed procedure has many advantages such as; high precision, long-term stability and enhanced sensitivity towards PFF compared other pesticide family members. As an application, the proposed sensor has been applied for determination of PFF in real vegetables and spiked water samples with satisfactory results.

Keywords

• Glassy carbon electrode
• Graphene oxide
• Imprinted polymer
• Profenofos