Analysis of miRNA-mRNA Crosstalk in Radiation-Induced Mouse Thymic Lymphomas to Identify miR-486 as a Critical Regulator by Targeting IGF2BP3 mRNA

Hainan Zhao; Hainan Zhao; Suhe Dong; Jicong Du; Penglin Xia; Ruling Liu; Tingting Liu; Yajie Yang; Ying Cheng; Jianming Cai; Cong Liu; Fu Gao; Hu Liu

doi:10.3389/fonc.2020.574001

Frontiers in Oncology (Feb 2021)

Analysis of miRNA-mRNA Crosstalk in Radiation-Induced Mouse Thymic Lymphomas to Identify miR-486 as a Critical Regulator by Targeting IGF2BP3 mRNA

Hainan Zhao,
Hainan Zhao,
Suhe Dong,
Jicong Du,
Penglin Xia,
Ruling Liu,
Tingting Liu,
Yajie Yang,
Ying Cheng,
Jianming Cai,
Cong Liu,
Fu Gao,
Hu Liu

Affiliations

Hainan Zhao: Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, China
Hainan Zhao: Department of Radiology Intervention, Changhai Hospital Affiliated to the Second Military Medical University, Shanghai, China
Suhe Dong: PLA Rocket Force Characteristic Medical Center, Beijing, China
Jicong Du: Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, China
Penglin Xia: Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, China
Ruling Liu: Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, China
Tingting Liu: Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, China
Yajie Yang: College of Basic Medicine, Second Military Medical University, Shanghai, China
Ying Cheng: Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, China
Jianming Cai: Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, China
Cong Liu: Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, China
Fu Gao: Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, China
Hu Liu: Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, China

DOI: https://doi.org/10.3389/fonc.2020.574001
Journal volume & issue: Vol. 10

Abstract

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Ionizing radiation is one of the common environmental carcinogens. miRNAs play critical roles in the processes of tumor occurrence, development, metastasis. However, the relationship between radiation-induced carcinogenesis and miRNA rarely reported. This study is aimed to investigate the effect of miRNAs on radiation-induced carcinogenesis. In this study we established the radiation-induced thymic lymphoma mice model. By using miRNA array of RTL tissue and predicting for miRNAs target genes, a miRNA-mRNA crosstalk network was established. Based on this network, we identified a critical miRNA, miR-486, which was the most down-regulated in the radiation-induced carcinogenesis. Then the function of miR-486 was confirmed by using knockout mice and cellular experiments. As a result, miR-486 could inhibit proliferation of mouse lymphoma cells by targeting IGF2BP3 mRNA. The adenovirus over-expression miR-486 vector reduced tumorigenesis in vivo. MiR-486 knockout mice have a strong tendency of radiation-induced carcinogenesis. In conclusion, miR-486 inhibits the proliferation of lymphoma cells and tumorigenesis induced by radiation through targeting IGF2BP3.

Published in Frontiers in Oncology

ISSN: 2234-943X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Medicine: Internal medicine: Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Website: https://www.frontiersin.org/journals/oncology/

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