Generation of a human ACTA1-tdTomato reporter iPSC line using CRISPR/Cas9 editing

Peter J. Houweling; Vanessa Crossman; Chrystal F. Tiong; Chantal A. Coles; Rhonda L. Taylor; Joshua S. Clayton; Alison Graham; Katerina Vlahos; Sara E Howden; Kathryn N. North

Stem Cell Research (Mar 2024)

Generation of a human ACTA1-tdTomato reporter iPSC line using CRISPR/Cas9 editing

Peter J. Houweling,
Vanessa Crossman,
Chrystal F. Tiong,
Chantal A. Coles,
Rhonda L. Taylor,
Joshua S. Clayton,
Alison Graham,
Katerina Vlahos,
Sara E Howden,
Kathryn N. North

Affiliations

Peter J. Houweling: Murdoch Children’s Research Institute, Melbourne, Victoria, Australia; Department of Pediatrics, The University of Melbourne, Victoria, Australia; Corresponding author at: Murdoch Children’s Research Institute, Melbourne, Victoria, Australia.
Vanessa Crossman: Murdoch Children’s Research Institute, Melbourne, Victoria, Australia
Chrystal F. Tiong: Murdoch Children’s Research Institute, Melbourne, Victoria, Australia
Chantal A. Coles: Murdoch Children’s Research Institute, Melbourne, Victoria, Australia; Department of Pediatrics, The University of Melbourne, Victoria, Australia
Rhonda L. Taylor: Harry Perkins Institute of Medical Research, QEII Medical Centre, Nedlands, WA, Australia; Centre for Medical Research, University of Western Australia, QEII Medical Centre, Nedlands, WA, Australia
Joshua S. Clayton: Harry Perkins Institute of Medical Research, QEII Medical Centre, Nedlands, WA, Australia; Centre for Medical Research, University of Western Australia, QEII Medical Centre, Nedlands, WA, Australia
Alison Graham: Murdoch Children’s Research Institute, Melbourne, Victoria, Australia
Katerina Vlahos: Murdoch Children’s Research Institute, Melbourne, Victoria, Australia
Sara E Howden: Murdoch Children’s Research Institute, Melbourne, Victoria, Australia
Kathryn N. North: Murdoch Children’s Research Institute, Melbourne, Victoria, Australia; Department of Pediatrics, The University of Melbourne, Victoria, Australia

Journal volume & issue: Vol. 75
p. 103313

Abstract

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We used gene editing to introduce DNA sequences encoding the tdTomato fluorescent protein into the α -skeletal actin 1 (ACTA1) locus to develop an ACTA1-tdTomato induced pluripotent stem cell reporter line for monitoring differentiation of skeletal muscle. This cell line will be used to better understand skeletal muscle maturation and development in vitro as well as provide a useful tool for drug screening and the evaluation of novel therapeutics for the treatment of skeletal muscle disease.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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