Проблемы особо опасных инфекций (Sep 2015)
Monoclonal Antibodies and Recombinant Proteins for Immune-Diagnostics of Ebola Virus Disease
Abstract
The review summarizes the literature data on the studies of viral antigens and antibodies, specific to Ebola virus, suitable for the development of a test-system for immune-diagnostics of the disease caused by them. Represented are the results of investigations on the obtainment of hybridoma monoclonal antibodies (MCA), specific to structural viral proteins NP, VP40, and VP35; recombinant proteins NP, VP40, and VP35 in prokaryotic system of E. coli; as well as the possibility to utilize MCA panels for EV viral and recombinant proteins’ detection in ELISA.
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