Communications Biology (Jan 2022)

Transfected plasmid DNA is incorporated into the nucleus via nuclear envelope reformation at telophase

  • Tokuko Haraguchi,
  • Takako Koujin,
  • Tomoko Shindo,
  • Şükriye Bilir,
  • Hiroko Osakada,
  • Kohei Nishimura,
  • Yasuhiro Hirano,
  • Haruhiko Asakawa,
  • Chie Mori,
  • Shouhei Kobayashi,
  • Yasushi Okada,
  • Yuji Chikashige,
  • Tatsuo Fukagawa,
  • Shinsuke Shibata,
  • Yasushi Hiraoka

DOI
https://doi.org/10.1038/s42003-022-03021-8
Journal volume & issue
Vol. 5, no. 1
pp. 1 – 15

Abstract

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Haraguchi et al. investigate how transfected DNA is incorporated into the nucleus using light and electron microscopy, and the LacI/LacO system. The authors report that cytoplasmic localised plasmid DNA is incorporated into the nucleus as it reforms during M-exit and transgene expression was achieved only after nuclear reformation in the next G1.