Cancer Management and Research (May 2020)
LncRNA PLAC 2 Is Downregulated in Osteosarcoma and Regulates Cancer Cell Proliferation Through miR-93
Abstract
Xiangran Sun, Ling Yu, Yubo Shi, Weichun Guo Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, People’s Republic of ChinaCorrespondence: Weichun GuoDepartment of Orthopedics, Renmin Hospital of Wuhan University, Hubei Zhang Road Wuchang District No. 99 Jiefang Road 238, Wuhan 430060, Hubei Province, People’s Republic of ChinaTel +86-27-88041911-82209Fax +86-27-88041911Email [email protected]: PLAC 2 is a tumor-suppressive lncRNA in glioma, while its roles in other types of cancer remain unclear. This study was carried out to explore the potential involvement of PLAC 2 in osteosarcoma (OS).Methods: Expression levels of PLAC 2 in OS and paired non-tumor tissues from OS patients were determined by RT-qPCR. A follow-up study was performed to analyze the prognostic value of PLAC 2 for OS. Interactions between PLAC 2 and miR-93 were assessed by cell transfection, followed by RT-qPCR. Cell proliferation assay was performed to analyze cell proliferation.Results: Our results showed that PLAC 2 was downregulated in OS tissues, and the high expression levels of PLAC 2 were associated with favorable overall survival of OS patients. MiR-93 was upregulated in OS tissues and its expression was inversely correlated with the expression of PLAC 2. In OS cells, overexpression of PLAC 2 resulted in downregulated miR-93, while overexpression of miR-93 did not affect the expression of PLAC 2. Overexpression of PLAC 2 led to decreased proliferation rate of OS cells, while overexpression of miR-93 showed opposite roles and reduced the overexpressing effects of PLAC 2.Conclusion: PLAC 2 is downregulated in OS and regulates cancer cell proliferation through miR-93.Keywords: osteosarcoma, PLAC 2, miR-93, survival, proliferation
