Structural basis of GABARAP-mediated GABAA receptor trafficking and functions on GABAergic synaptic transmission

Jin Ye; Guichang Zou; Ruichi Zhu; Chao Kong; Chenjian Miao; Mingjie Zhang; Jianchao Li; Wei Xiong; Chao Wang

doi:10.1038/s41467-020-20624-z

Nature Communications (Jan 2021)

Structural basis of GABARAP-mediated GABAA receptor trafficking and functions on GABAergic synaptic transmission

Jin Ye,
Guichang Zou,
Ruichi Zhu,
Chao Kong,
Chenjian Miao,
Mingjie Zhang,
Jianchao Li,
Wei Xiong,
Chao Wang

Affiliations

Jin Ye: MOE Key Laboratory for Membraneless Organelles & Cellular Dynamics, Hefei National Laboratory for Physical Sciences at the Microscale, School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China
Guichang Zou: MOE Key Laboratory for Membraneless Organelles & Cellular Dynamics, Hefei National Laboratory for Physical Sciences at the Microscale, School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China
Ruichi Zhu: Division of Life Science, State Key Laboratory of Molecular Neuroscience, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon
Chao Kong: MOE Key Laboratory for Membraneless Organelles & Cellular Dynamics, Hefei National Laboratory for Physical Sciences at the Microscale, School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China
Chenjian Miao: MOE Key Laboratory for Membraneless Organelles & Cellular Dynamics, Hefei National Laboratory for Physical Sciences at the Microscale, School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China
Mingjie Zhang: Division of Life Science, State Key Laboratory of Molecular Neuroscience, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon
Jianchao Li: Division of Cell, Developmental and Integrative Biology, School of Medicine, South China University of Technology
Wei Xiong: MOE Key Laboratory for Membraneless Organelles & Cellular Dynamics, Hefei National Laboratory for Physical Sciences at the Microscale, School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China
Chao Wang: MOE Key Laboratory for Membraneless Organelles & Cellular Dynamics, Hefei National Laboratory for Physical Sciences at the Microscale, School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China

DOI: https://doi.org/10.1038/s41467-020-20624-z
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 12

Abstract

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The GABARAP protein is known to support the stability of GABAA receptors (GABAARs) in synapses, but the underlying molecular mechanisms remained to be elucidated. Here authors use biochemistry, X-ray crystallography and electrophsyiology and show that GABARAP directly binds to a previously unappreciated region in the γ2 subunit of GABAAR.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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