Acta Medica Leopoliensia (Dec 2019)

Dynamics of laboratory indicators of rats after implantation of bioglass material and glass ceramics

  • V. Shymon,
  • S. Alfeldii,
  • M. Shymon,
  • V. Stoyka

DOI
https://doi.org/10.25040/aml2019.04.045
Journal volume & issue
Vol. 25, no. 4
pp. 45 – 49

Abstract

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Aim. To explore the recovery of the structure and density of the spongy and cortical bone after filling the defect with the bioglass developed by the authors. Material and Methods. The experiment was performed on white rats where the dynamics of blood biochemical markers were studied after implantation of glass-based and glass-ceramic materials. The study rats were divided into 2 groups. In the first group a material on the basis of glass ceramics AC-5 was implanted; in the second group - a material on the basis of glass FAR-5. Implant toxicity was determined based on the study of the dynamics of serum protein content, urea and alanine aminotransferase (ALT) enzyme activity. The state of restoration of the structure and density of the spongy and cortical bone was examined using alkaline phosphatase level. Results and Discussion. The study found no impact of implants based on glass ceramics AC-5 on the functional state of the liver and kidneys of experimental animals, but revealed the presence of inflammatory-regenerative changes in bone tissue at the implant site manifested only on day 7 of observation, which is normal regeneration after implantation. The dynamics of alkaline phosphatase activity in the serum of rats corresponded to the stages of bone regeneration and implant survival. In the rats of the second group, the content of total protein, unlike the animals of the first group, was significantly higher compared to the intact rats at all observation periods, indicating the presence of intoxication in animals. The glycoprotein content was increased, indicating the presence of inflammation in the animals at the end of the study. In contrast to the rats of the first group, the activity of alkaline phosphatase was significantly increased compared with that of the intact rats. The indicator increased with the observation period and returned to the reference values by the 90th day. Conclusion. Based on the study of the markers of bone metabolism (alkaline phosphatase activity and serum level of chondroitin sulfate ), a longer process of bone remodeling in case of implantation of the FAR-5 implant material was revealed, which increased compared to the test parameters of the intact animals.

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