The significant clinical correlation of the intratumor oral microbiome in oral squamous cell carcinoma based on tissue-derived sequencing

Zhengrui Li; Zhengrui Li; Zhengrui Li; Zhengrui Li; Zhengrui Li; Zhengrui Li; Rao Fu; Rao Fu; Rao Fu; Rao Fu; Rao Fu; Rao Fu; Xutao Wen; Xutao Wen; Xutao Wen; Xutao Wen; Xutao Wen; Xutao Wen; Qi Wang; Xufeng Huang; Ling Zhang; Ling Zhang; Ling Zhang; Ling Zhang; Ling Zhang; Ling Zhang

doi:10.3389/fphys.2022.1089539

Frontiers in Physiology (Jan 2023)

The significant clinical correlation of the intratumor oral microbiome in oral squamous cell carcinoma based on tissue-derived sequencing

Zhengrui Li,
Zhengrui Li,
Zhengrui Li,
Zhengrui Li,
Zhengrui Li,
Zhengrui Li,
Rao Fu,
Rao Fu,
Rao Fu,
Rao Fu,
Rao Fu,
Rao Fu,
Xutao Wen,
Xutao Wen,
Xutao Wen,
Xutao Wen,
Xutao Wen,
Xutao Wen,
Qi Wang,
Xufeng Huang,
Ling Zhang,
Ling Zhang,
Ling Zhang,
Ling Zhang,
Ling Zhang,
Ling Zhang

Affiliations

Zhengrui Li: Department of Oral and Maxillofacial-Head and Neck Oncology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Zhengrui Li: College of Stomatology, Shanghai Jiao Tong University, Shanghai, China
Zhengrui Li: National Center for Stomatology, Shanghai, China
Zhengrui Li: National Clinical Research Center for Oral Diseases, Shanghai, China
Zhengrui Li: Shanghai Key Laboratory of Stomatology, Shanghai, China
Zhengrui Li: Shanghai Research Institute of Stomatology, Shanghai, China
Rao Fu: Department of Oral and Maxillofacial-Head and Neck Oncology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Rao Fu: College of Stomatology, Shanghai Jiao Tong University, Shanghai, China
Rao Fu: National Center for Stomatology, Shanghai, China
Rao Fu: National Clinical Research Center for Oral Diseases, Shanghai, China
Rao Fu: Shanghai Key Laboratory of Stomatology, Shanghai, China
Rao Fu: Shanghai Research Institute of Stomatology, Shanghai, China
Xutao Wen: Department of Oral and Maxillofacial-Head and Neck Oncology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Xutao Wen: College of Stomatology, Shanghai Jiao Tong University, Shanghai, China
Xutao Wen: National Center for Stomatology, Shanghai, China
Xutao Wen: National Clinical Research Center for Oral Diseases, Shanghai, China
Xutao Wen: Shanghai Key Laboratory of Stomatology, Shanghai, China
Xutao Wen: Shanghai Research Institute of Stomatology, Shanghai, China
Qi Wang: Department of Gastroenterology, Affliated Hospital of Jiangsu University, Jiangsu University, Zhenjiang, China
Xufeng Huang: Faculty of Dentistry, University of Debrecen, Debrecen, Hungary
Ling Zhang: Department of Oral and Maxillofacial-Head and Neck Oncology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Ling Zhang: College of Stomatology, Shanghai Jiao Tong University, Shanghai, China
Ling Zhang: National Center for Stomatology, Shanghai, China
Ling Zhang: National Clinical Research Center for Oral Diseases, Shanghai, China
Ling Zhang: Shanghai Key Laboratory of Stomatology, Shanghai, China
Ling Zhang: Shanghai Research Institute of Stomatology, Shanghai, China

DOI: https://doi.org/10.3389/fphys.2022.1089539
Journal volume & issue: Vol. 13

Abstract

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Background: The microbiota is a critical component of the complex human microenvironment, impacting various physiological processes and disease development via the microbe–host interaction. In particular, the oral microbiota profoundly affects tumor development and progression. There is increasing evidence that oral microbiota is associated with the development of oral cancer, especially oral squamous cell carcinoma (OSCC).Methods: We comprehensively analyzed the oral microbiota in 133 OSCC samples worldwide. Subsequently, we evaluated the microbial compositions between OSCC patients and healthy people and their correlation with clinical parameters. The value of the oral microbiota as a diagnostic and prognostic biomarker was also determined.Results: This study found differences in critical oral microbiota between OSCC and normal controls. The most notable differences are present in p_Firmicutes, p_Actinobacteria, c_Fusobacteriia, o_Fusobacteriales, f_Fusobacteriaceae, and g_Fusobacterium. All six-level oral microorganisms were also associated with the clinical characteristics of OSCC, particularly with the clinical outcomes (survival time and status). We developed a predictive model based on this. We found that five different oral microorganisms have high confidence and can be used for clinical diagnosis and prognostic prediction, except for p_Actinobacteria.Conclusion: This study revealed that the intratumor oral microbiota of OSCC patients worldwide and the microbial signatures of OSCC patients possess similar properties in different regions, further refining the shortcomings of the current research field. We revealed that the oral microbiota could be used as a biomarker to reflect human health and disease progression status. This will provide new directions for tumor microbiome research. This means we can develop strategies through diet, probiotics, and antibiotics for cancer prevention and treatment.

Published in Frontiers in Physiology

ISSN: 1664-042X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Physiology
Website: https://www.frontiersin.org/journals/physiology

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