酶法制备甘油二酯的研究进展Review on progress of enzymatic production of diacylglycerol

Abstract

甘油二酯（DAG）可抑制体内脂肪堆积、降低血清甘油三酯水平从而具有减肥效果。与化学法相比，酶法制备DAG具有反应条件温和、选择性强、环境友好等优点。综述了国内外酶法制备DAG的研究现状，对酶促酯化合成、酶促甘油解以及酶促水解法制备DAG进行了详细介绍，着重阐述了如何提高DAG的选择性，总结了脂肪酶在DAG制备中的关键问题，以期为DAG的工业化生产提供参考。 Diacylglycerol(DAG) can reduce weight due to its ability of inhibiting fat accumulating and reducing the level of triacylglycerol(TAG). Compared with chemical catalysis, enzymatic catalysis of DAG production exhibits mild reaction conditions, higher selectivity and environmental friendly. The status of enzymatic production of DAG in worldwide was reviewed. Preparation of DAG by enzymatic esterification synthesis, enzymatic glycerolysis and enzymatic hydrolysis was introduced in details. Besides, how to improve the specificity of DAG was emphasized and the key problems of lipase in the preparation of DAG were summarized, so as to provide reference for the industrial production of DAG.

Keywords

• 脂肪酶；甘油二酯；酯化；甘油解；水解
• lipase; diacylglycerol; esterification; glycerolysis; hydrolysis