Nature Communications (Nov 2016)

TruePrime is a novel method for whole-genome amplification from single cells based on TthPrimPol

  • Ángel J. Picher,
  • Bettina Budeus,
  • Oliver Wafzig,
  • Carola Krüger,
  • Sara García-Gómez,
  • María I. Martínez-Jiménez,
  • Alberto Díaz-Talavera,
  • Daniela Weber,
  • Luis Blanco,
  • Armin Schneider

DOI
https://doi.org/10.1038/ncomms13296
Journal volume & issue
Vol. 7, no. 1
pp. 1 – 16

Abstract

Read online

Single cell genomic analysis needs DNA amplification with high fidelity and accuracy. Here, the authors devise a novel multiple displacement amplification method called TruePrime that is based in Thermus thermophilusPrimPol and Phi29 DNA polymerase, and demonstrate its utility and accuracy.