Synthesis and Characterization of Thymine-15N2

Abstract

A novel and mild synthesis method of thymine-15N2 was designed. Ethyl formate and ethyl propionate as raw materials, the intermediate ethyl 2-formylpropionate was condensed under sodium metal, the target compound thymine-15N2 was obtained by condensation with isotope urea-15N2 under acid catalysis. Synthesis route had the advantages of simple operation, short process flow and less side product. The yield was more than 80% and isotope 15N abundance did not diluted. The product was characterized by HPLC, IR, MS, 1H NMR and 13C NMR. The chemical purity was more than 99% and 15N enrichment was more than 98 atom%.

Keywords

• isotope
• thymine
• ethyl 2-formylpropionate