Cytotoxic, Antitumor and Toxicological Profile of <i>Passiflora alata</i> Leaf Extract
Ricardo G. Amaral,
Silvana V. F. Gomes,
Luciana N. Andrade,
Sara A. dos Santos,
Patrícia Severino,
Ricardo L. C. de Albuquerque Júnior,
Eliana B. Souto,
Geraldo C. Brandão,
Sandra L. Santos,
Jorge M. David,
Adriana A. Carvalho
Affiliations
Ricardo G. Amaral
Department of Physiology, Federal University of Sergipe, São Cristóvão, Sergipe 49100-000, Brazil
Silvana V. F. Gomes
Institute of Technology and Research, University of Tiradentes, Aracaju, Sergipe 49032-490, Brazil
Luciana N. Andrade
Department of Medicine, Federal University of Sergipe (UFS), Avenida Governador Marcelo Déda, Lagarto-SE 49400-000, Brazil
Sara A. dos Santos
Department of Physiology, Federal University of Sergipe, São Cristóvão, Sergipe 49100-000, Brazil
Patrícia Severino
Institute of Technology and Research, University of Tiradentes, Aracaju, Sergipe 49032-490, Brazil
Ricardo L. C. de Albuquerque Júnior
Institute of Technology and Research, University of Tiradentes, Aracaju, Sergipe 49032-490, Brazil
Eliana B. Souto
Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Coimbra, Pólo das Ciências da Saúde, Azinhaga de Santa Comba, 3000-548 Coimbra, Portugal
Geraldo C. Brandão
Department of Pharmacy, Federal University of Ouro Preto, Ouro Preto 78950-000, Brazil
Sandra L. Santos
Department of Physiology, Federal University of Sergipe, São Cristóvão, Sergipe 49100-000, Brazil
Jorge M. David
Institute of Chemistry, Federal University of Bahia, Salvador 40000-000, Brazil
Adriana A. Carvalho
Department of Medicine, Federal University of Sergipe (UFS), Avenida Governador Marcelo Déda, Lagarto-SE 49400-000, Brazil
Passiflora alata or passion fruit is a native flowering plant from Amazon, geographically spread from Peru to Brazil. The plant has long been used in folks medicine for its pharmacological properties and is included in the Brazilian Pharmacopoeia since 1929. The aim of this study was to evaluate the potential cytotoxic and antitumor activities of Passiflora alata leaf extract (PaLE) in S180-tumor bearing mice. The percentage of cell proliferation inhibition (% CPI) and IC50 in relation to 4 tumor cell lines were determined in PC3, K-562, HepG2 and S180 cell lines using the MTT assay. PaLE showed a CPI > 75% and greater potency (IC50 PaLE showed antitumor activity in treatments intraperitoneally (36.75% and 44.99% at doses of 100 and 150 mg/kg/day, respectively). Toxicological changes were shown in the reduced body mass associated with reduced food consumption, increased spleen mass associated with histopathological increase in the white pulp of the spleen and increased number of total leukocytes with changes in the percentage relationship between lymphocytes and neutrophils. Our outcomes corroborate the conclusion that PaLE has antitumor activity in vitro and in vivo with low toxicity.
