Dynamic Transcriptome Profiling of Mungbean Genotypes Unveil the Genes Respond to the Infection of Mungbean Yellow Mosaic Virus
Manickam Sudha,
Adhimoolam Karthikeyan,
Balasubramaniam Madhumitha,
Rajagopalan Veera Ranjani,
Mayalagu Kanimoli Mathivathana,
Manickam Dhasarathan,
Jayakodi Murukarthick,
Madiha Natchi Samu Shihabdeen,
Karuppiah Eraivan Arutkani Aiyanathan,
Muthaiyan Pandiyan,
Natesan Senthil,
Muthurajan Raveendran
Affiliations
Manickam Sudha
Department of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore 641003, Tamil Nadu, India
Adhimoolam Karthikeyan
Department of Biotechnology, Centre of Innovation, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Madurai 625104, Tamil Nadu, India
Balasubramaniam Madhumitha
Department of Plant Pathology, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Madurai 625104, Tamil Nadu, India
Rajagopalan Veera Ranjani
Department of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore 641003, Tamil Nadu, India
Mayalagu Kanimoli Mathivathana
Department of Plant Breeding and Genetics, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Madurai 625104, Tamil Nadu, India
Manickam Dhasarathan
Agroclimate Research Centre, Directorate of Crop Management, Tamil Nadu Agricultural University, Coimbatore 641003, Tamil Nadu, India
Jayakodi Murukarthick
Gene Bank, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Stadt See land, 06466 Seeland, OT Gatersleben, Germany
Madiha Natchi Samu Shihabdeen
Department of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore 641003, Tamil Nadu, India
Karuppiah Eraivan Arutkani Aiyanathan
Agricultural College and Research Institute, Tamil Nadu Agricultural University, Killikulam 628252, Tamil Nadu, India
Muthaiyan Pandiyan
Regional Research Station, Tamil Nadu Agricultural University, Virudhachalam 606001, Tamil Nadu, India
Natesan Senthil
Department of Plant Molecular Biology and Bioinformatics, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore 641003, Tamil Nadu, India
Muthurajan Raveendran
Department of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore 641003, Tamil Nadu, India
Yellow mosaic disease (YMD), incited by mungbean yellow mosaic virus (MYMV), is a primary viral disease that reduces mungbean production in South Asia, especially in India. There is no detailed knowledge regarding the genes and molecular mechanisms conferring resistance of mungbean to MYMV. Therefore, disclosing the genetic and molecular bases related to MYMV resistance helps to develop the mungbean genotypes with MYMV resistance. In this study, transcriptomes of mungbean genotypes, VGGRU-1 (resistant) and VRM (Gg) 1 (susceptible) infected with MYMV were compared to those of uninfected controls. The number of differentially expressed genes (DEGs) in the resistant and susceptible genotypes was 896 and 506, respectively. Among them, 275 DEGs were common between the resistant and susceptible genotypes. Functional annotation of DEGs revealed that the DEGs belonged to the following categories defense and pathogenesis, receptor-like kinases; serine/threonine protein kinases, hormone signaling, transcription factors, and chaperons, and secondary metabolites. Further, we have confirmed the expression pattern of several DEGs by quantitative real-time PCR (qRT-PCR) analysis. Collectively, the information obtained in this study unveils the new insights into characterizing the MYMV resistance and paved the way for breeding MYMV resistant mungbean in the future.
