Karyological and molecular studies between six species of Plantago in the Northern border region at Saudi Arabia

Abstract

Karyotype characters, mitotic metaphase chromosomes, and haploid ideograms of six species of Plantago L. species. Analysis of somatic metaphases showed that the chromosome numbers of these species were 2n = 8 for P. ovata, 2n = 10 for P. albicans, P. amplexicaulis, P. ciliata and P. psammophilia, and 2n = 12 for P. lanceolata. The karyotype formulae were nsm (+), nsm (–) and nm. According to TF% and Syi index, and according to A and A1, P. ciliata may be considered the most symmetrical and P. lanceolata may be had the most asymmetrical karyotype. There are 63 total bands and 11unique bands resulting from RAPD; 23 total bands and 3unique bands resulting from both ISSR and 23 total bands in addition to 2 unique bands generated from SCoT markers. Perceptual mapping (Bilpot) and cluster analysis based on cytological and molecular attributes gave the same results that distinguished the studied taxa into two main groups; P. ovata and the other taxa also classified into two subgroups.

Keywords

• plantago
• karyotype
• ideogram
• rapd
• issr
• scot