Comparative analysis of two paradigm bacteriophytochromes reveals opposite functionalities in two-component signaling

Elina Multamäki; Rahul Nanekar; Dmitry Morozov; Topias Lievonen; David Golonka; Weixiao Yuan Wahlgren; Brigitte Stucki-Buchli; Jari Rossi; Vesa P. Hytönen; Sebastian Westenhoff; Janne A. Ihalainen; Andreas Möglich; Heikki Takala

doi:10.1038/s41467-021-24676-7

Nature Communications (Jul 2021)

Comparative analysis of two paradigm bacteriophytochromes reveals opposite functionalities in two-component signaling

Elina Multamäki,
Rahul Nanekar,
Dmitry Morozov,
Topias Lievonen,
David Golonka,
Weixiao Yuan Wahlgren,
Brigitte Stucki-Buchli,
Jari Rossi,
Vesa P. Hytönen,
Sebastian Westenhoff,
Janne A. Ihalainen,
Andreas Möglich,
Heikki Takala

Affiliations

Elina Multamäki: Faculty of Medicine, Anatomy, University of Helsinki
Rahul Nanekar: Department of Biological and Environmental Science, Nanoscience Center, University of Jyvaskyla
Dmitry Morozov: Department of Chemistry, Nanoscience Center, University of Jyvaskyla
Topias Lievonen: Department of Biological and Environmental Science, Nanoscience Center, University of Jyvaskyla
David Golonka: Lehrstuhl für Biochemie, Universität Bayreuth
Weixiao Yuan Wahlgren: Department of Chemistry and Molecular Biology, University of Gothenburg
Brigitte Stucki-Buchli: Department of Biological and Environmental Science, Nanoscience Center, University of Jyvaskyla
Jari Rossi: Faculty of Medicine, Anatomy, University of Helsinki
Vesa P. Hytönen: Faculty of Medicine and Health Technology, BioMediTech, Tampere University
Sebastian Westenhoff: Department of Chemistry and Molecular Biology, University of Gothenburg
Janne A. Ihalainen: Department of Biological and Environmental Science, Nanoscience Center, University of Jyvaskyla
Andreas Möglich: Lehrstuhl für Biochemie, Universität Bayreuth
Heikki Takala: Faculty of Medicine, Anatomy, University of Helsinki

DOI: https://doi.org/10.1038/s41467-021-24676-7
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 14

Abstract

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The bacteriophytochrome DrBphP from Deinococcus radiodurans shows high sequence homology to the histidine kinase Agp1 from Agrobacterium fabrum but lacks kinase activity. Here, the authors structurally and biochemically analyse DrBphP and Agp1, showing that DrBphP is a light-activatable phosphatase.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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