Bioactive Coatings Based on Hydroxyapatite, Kanamycin, and Growth Factor for Biofilm Modulation
Oana Gherasim,
Alexandru Mihai Grumezescu,
Valentina Grumezescu,
Irina Negut,
Marius Florin Dumitrescu,
Miruna Silvia Stan,
Ionela Cristina Nica,
Alina Maria Holban,
Gabriel Socol,
Ecaterina Andronescu
Affiliations
Oana Gherasim
Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, Politehnica University of Bucharest, 011061 Bucharest, Romania
Alexandru Mihai Grumezescu
Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, Politehnica University of Bucharest, 011061 Bucharest, Romania
Valentina Grumezescu
Lasers Department, National Institute for Lasers, Plasma and Radiation Physics, 077125 Magurele, Romania
Irina Negut
Lasers Department, National Institute for Lasers, Plasma and Radiation Physics, 077125 Magurele, Romania
Marius Florin Dumitrescu
Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, Politehnica University of Bucharest, 011061 Bucharest, Romania
Miruna Silvia Stan
Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, Politehnica University of Bucharest, 011061 Bucharest, Romania
Ionela Cristina Nica
Research Institute of the University of Bucharest–ICUB, University of Bucharest, 050657 Bucharest, Romania
Alina Maria Holban
Research Institute of the University of Bucharest–ICUB, University of Bucharest, 050657 Bucharest, Romania
Gabriel Socol
Lasers Department, National Institute for Lasers, Plasma and Radiation Physics, 077125 Magurele, Romania
Ecaterina Andronescu
Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, Politehnica University of Bucharest, 011061 Bucharest, Romania
The occurrence of opportunistic local infections and improper integration of metallic implants results in severe health conditions. Protective and tunable coatings represent an attractive and challenging selection for improving the metallic devices’ biofunctional performances to restore or replace bone tissue. Composite materials based on hydroxyapatite (HAp), Kanamycin (KAN), and fibroblast growth factor 2 (FGF2) are herein proposed as multifunctional coatings for hard tissue implants. The superior cytocompatibility of the obtained composite coatings was evidenced by performing proliferation and morphological assays on osteoblast cell cultures. The addition of FGF2 proved beneficial concerning the metabolic activity, adhesion, and spreading of cells. The KAN-embedded coatings exhibited significant inhibitory effects against bacterial biofilm development for at least two days, the results being superior in the case of Gram-positive pathogens. HAp-based coatings embedded with KAN and FGF2 protein are proposed as multifunctional materials with superior osseointegration potential and the ability to reduce device-associated infections.
