Nature Communications (Jul 2022)

A plasmid system with tunable copy number

  • Miles V. Rouches,
  • Yasu Xu,
  • Louis Brian Georges Cortes,
  • Guillaume Lambert

DOI
https://doi.org/10.1038/s41467-022-31422-0
Journal volume & issue
Vol. 13, no. 1
pp. 1 – 12

Abstract

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The range of available copy numbers for cloning vectors is largely restricted to the handful of ORIs that have been isolated from plasmids found in nature. Here the authors introduce a plasmid system that allow for the continuous, finely-tuned control of plasmid copy number between 1 and 800 copies per cell.