Aktualʹnì Pitannâ Farmacevtičnoï ì Medičnoï Nauki ta Praktiki (Aug 2019)
Production technology and chromato-mass spectroscopy of the valeriana officinalis tinctures
Abstract
Underground organs of Valeriana Officinalis are part of multicomponent phytocompositions, which allows them to vary the content and, thus, intensively affect various links of pathological disorders in the body. Despite this, valeriana drug remains poorly understood because of its extraordinary polymorphism. The purpose of the work is to determine, by means of gas-liquid chromatography, the component composition of valerian tinctures produced by PrAT Pharmaceutical Factory “Viola”, made from rhizomes with valerian roots from different suppliers / manufacturers and various series. Materials and methods. For experimental studies we took samples of tinctures of rhizomes with valerian roots produced by PRAT Pharmaceutical Factory Viola, made from medicinal plant raw materials of valerian from different suppliers / producers, namely: from firm “Herbar Sp.zoo”, Warsaw, Poland and from firm LLC “Fitosvit Ltd”, Vinnitsa region, Ukraine according to the traditional formula (Tinctura Rhizomata cum radicibus Valerianae (1: 5). Component composition of the valeriana tinctures was investigated using the gas chromatograph Agilent 7890B with a 5977B mass spectrometry detector. Results. The component composition the of the valerian tinctures was identified by chromatography-mass spectrometry. In the analysis of the chromatogram of the four valerian tinctures series, 75 components were identified, 25 of which were present in all series of studied tinctures. It should be noted that six compounds (3, 14, 36, 40, 47, and 52) in all series are present in large quantities. Among them valerian acid (compound 47), the content of which in three series is almost at the same level of 3.1–3.72 %. The smallest amount is contained in the tincture of valerian from the raw materials of LLC "Fitosvit Ltd" 150518 series and is 1.54 %, and the biggest amount in the tincture by firm "Herbar" series 040118–3.72 %. Conclusions. Analyzing the obtained data of the GLC it can be noted that valerian tinctures differ both on quantitative and qualitative composition. 25 compounds are present in all investigated series, 6 of which are present in large amounts. Since the tinctures were made in one technology, the content of essential oils in the medicinal plant depends by the place, time of collection, drying of raw materials, ecology, climatic latitudes. Deeper pharmacological and pharmacological research is needed on the territory of Ukraine.
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