Ozone-Induced Lung and Bronchial Injury: A Mouse Model Study
Shi Liang,
Yan Sha,
Chuanhong Yang,
Huangwen Lai,
Chong Sun,
Weisen Zhao,
An Zhang,
Qingwen Qi,
Ying Xie
Affiliations
Shi Liang
National Key Clinical Specialty of Occupational Diseases, Shenzhen Prevention and Treatment Center for Occupational Diseases, Shenzhen 518020, China
Yan Sha
National Key Clinical Specialty of Occupational Diseases, Shenzhen Prevention and Treatment Center for Occupational Diseases, Shenzhen 518020, China
Chuanhong Yang
Laboratory of Basic Medical Science, Liuhuaqiao Hospital, Guangzhou 510010, China
Huangwen Lai
Laboratory of Basic Medical Science, Liuhuaqiao Hospital, Guangzhou 510010, China
Chong Sun
National Key Clinical Specialty of Occupational Diseases, Shenzhen Prevention and Treatment Center for Occupational Diseases, Shenzhen 518020, China
Weisen Zhao
Department of Occupational and Environmental Health, School of Public Health, Jilin University, Changchun 130021, China
An Zhang
National Key Laboratory of Resources and Environmental Information System, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China
Qingwen Qi
National Key Laboratory of Resources and Environmental Information System, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China
Ying Xie
National Key Clinical Specialty of Occupational Diseases, Shenzhen Prevention and Treatment Center for Occupational Diseases, Shenzhen 518020, China
Ozone pollution is a prominent public health issue, but there are few studies on the effect of ozone on the ultrastructure of respiratory system; we conducted this research. Exposed to 1.1 ppm O3 4 h per day, the mice lungs and bronchi were taken on the 15th or 30th day. The sections stained with HE and immunohistochemical streptavidin–peroxidase methods for NQO1, Nrf2, and Keap1 were observed and measured under the optical microscope. TEM was used for ultrastructure observation. The animals’ serums were detected for CRP and IL-6 levels. The HE-stained sections showed no obvious micromorphological changes in the O3 exposure, but the NQO1 average optical density was higher than the control on the 15th day (p 3 exposure group, such as bulges and vacuoles in type I alveolar cells, the increased evacuation of substance from lamellar bodies in the type II alveolar cells, the increased space around the goblet nucleus, binuclear goblet, and columnar cells. CRP and IL-6 levels increased compared with the control (p 3 had no significant effect on the micromorphology of the mice lungs and bronchi, it did affect the ultrastructure with oxidative stress and inflammatory responses.
