Медицинская иммунология (Jan 2025)
Experimental study of the effects of benz(a)pyrene and Chlorella growth factor in vivo associated with cellular clusters of innate and adaptive immunity
Abstract
Benz(a)pyrene is a first hazard class aromatic compound which exerts carcinogenic, mutagenic, hematotoxic, and immunotropic effects. The study of cell clusters influenced in vivo by exogenous haptens using laboratory mice as a model object allows us to expand knowledge about the mechanisms and features of immunotropic effects induced by benzo(a)pyrene. The experimental series involved 12 female outbred white mice. The concentration of benzo(a)pyrene for a single dose was 6 μg/L, To neutralize its effects, the Chlorella growth factor (CGF) concentrate was used as an adaptogen, which was consistently administered over 4 weeks at a dose of 60-70 mlns/mL of living cells. Both substances were administered via oral tube in a volume of 1 mL. The study of cell phagocytosis was carried out by the method of V.N. Kaplin. Analysis of the total number of leukocytes and lymphocytes was carried out using a hematology analyzer. The study of cell differentiation clusters was carried out using flow cytometry. Microsoft® Office Excel and Statistica 6.0 programs were used for statistical evaluation. Results: subchronic intoxication with benzo(a) pyrene under the in vivo experimental conditions led to significant modification of cellular immunity, exhibiting a phenotype of deficient innate cellular immunity factors, and imbalance of adaptive cellular immunity with a predominant inhibition of apoptotic activity (CD95+) and modeling of integrin- and VEGFmediated events (CD11a, CD309). Sequential intake of the natural modifier (CGF) was characterized by normalization of phagocytic activity and alleviation of benzo(a)pyrene-induced effects. Conclusions of the study are limited by small number of experimental sample, The in vivo experiment have shown an imbalanced pattern of nonspecific and adaptive cellular immunity, associated with granulocyte changes caused by benzo(a)pyrene along with disturbed control of cell proliferation alleviated by CGF. The intake of the CGF complex led to optimization of immune system as shown by a number of indexes, along with positively modified immunotropic effects of benzo(a)pyrene with cancelled suppression of granulocytic and integrin-associated lymphoid lineages, which provides, mostly, phagocytosis protection, as well as antiapoptotic and integrin-mimetic effects.
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