Frontiers in Physiology (Oct 2019)
Post-treatment With Qing-Ying-Tang, a Compound Chinese Medicine Relives Lipopolysaccharide-Induced Cerebral Microcirculation Disturbance in Mice
- Hao-Min Wang,
- Hao-Min Wang,
- Hao-Min Wang,
- Hao-Min Wang,
- Hao-Min Wang,
- Hao-Min Wang,
- Ping Huang,
- Ping Huang,
- Ping Huang,
- Ping Huang,
- Ping Huang,
- Quan Li,
- Quan Li,
- Quan Li,
- Quan Li,
- Quan Li,
- Lu-Lu Yan,
- Lu-Lu Yan,
- Lu-Lu Yan,
- Lu-Lu Yan,
- Lu-Lu Yan,
- Kai Sun,
- Kai Sun,
- Kai Sun,
- Kai Sun,
- Kai Sun,
- Li Yan,
- Li Yan,
- Li Yan,
- Li Yan,
- Li Yan,
- Chun-Shui Pan,
- Chun-Shui Pan,
- Chun-Shui Pan,
- Chun-Shui Pan,
- Chun-Shui Pan,
- Xiao-Hong Wei,
- Xiao-Hong Wei,
- Xiao-Hong Wei,
- Xiao-Hong Wei,
- Xiao-Hong Wei,
- Yu-Ying Liu,
- Yu-Ying Liu,
- Yu-Ying Liu,
- Yu-Ying Liu,
- Yu-Ying Liu,
- Bai-He Hu,
- Bai-He Hu,
- Bai-He Hu,
- Bai-He Hu,
- Bai-He Hu,
- Chuan-She Wang,
- Chuan-She Wang,
- Chuan-She Wang,
- Chuan-She Wang,
- Chuan-She Wang,
- Chuan-She Wang,
- Jing-Yu Fan,
- Jing-Yu Fan,
- Jing-Yu Fan,
- Jing-Yu Fan,
- Jing-Yu Fan,
- Jing-Yan Han,
- Jing-Yan Han,
- Jing-Yan Han,
- Jing-Yan Han,
- Jing-Yan Han,
- Jing-Yan Han
Affiliations
- Hao-Min Wang
- Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing, China
- Hao-Min Wang
- Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China
- Hao-Min Wang
- Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Beijing, China
- Hao-Min Wang
- Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Beijing, China
- Hao-Min Wang
- State Key Laboratory of Core Technology in Innovative Chinese Medicine, Beijing, China
- Hao-Min Wang
- Beijing Microvascular Institute of Integration of Chinese and Western Medicine, Beijing, China
- Ping Huang
- Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China
- Ping Huang
- Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Beijing, China
- Ping Huang
- Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Beijing, China
- Ping Huang
- State Key Laboratory of Core Technology in Innovative Chinese Medicine, Beijing, China
- Ping Huang
- Beijing Microvascular Institute of Integration of Chinese and Western Medicine, Beijing, China
- Quan Li
- Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China
- Quan Li
- Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Beijing, China
- Quan Li
- Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Beijing, China
- Quan Li
- State Key Laboratory of Core Technology in Innovative Chinese Medicine, Beijing, China
- Quan Li
- Beijing Microvascular Institute of Integration of Chinese and Western Medicine, Beijing, China
- Lu-Lu Yan
- Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China
- Lu-Lu Yan
- Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Beijing, China
- Lu-Lu Yan
- Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Beijing, China
- Lu-Lu Yan
- State Key Laboratory of Core Technology in Innovative Chinese Medicine, Beijing, China
- Lu-Lu Yan
- Beijing Microvascular Institute of Integration of Chinese and Western Medicine, Beijing, China
- Kai Sun
- Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China
- Kai Sun
- Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Beijing, China
- Kai Sun
- Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Beijing, China
- Kai Sun
- State Key Laboratory of Core Technology in Innovative Chinese Medicine, Beijing, China
- Kai Sun
- Beijing Microvascular Institute of Integration of Chinese and Western Medicine, Beijing, China
- Li Yan
- Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China
- Li Yan
- Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Beijing, China
- Li Yan
- Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Beijing, China
- Li Yan
- State Key Laboratory of Core Technology in Innovative Chinese Medicine, Beijing, China
- Li Yan
- Beijing Microvascular Institute of Integration of Chinese and Western Medicine, Beijing, China
- Chun-Shui Pan
- Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China
- Chun-Shui Pan
- Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Beijing, China
- Chun-Shui Pan
- Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Beijing, China
- Chun-Shui Pan
- State Key Laboratory of Core Technology in Innovative Chinese Medicine, Beijing, China
- Chun-Shui Pan
- Beijing Microvascular Institute of Integration of Chinese and Western Medicine, Beijing, China
- Xiao-Hong Wei
- Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China
- Xiao-Hong Wei
- Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Beijing, China
- Xiao-Hong Wei
- Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Beijing, China
- Xiao-Hong Wei
- State Key Laboratory of Core Technology in Innovative Chinese Medicine, Beijing, China
- Xiao-Hong Wei
- Beijing Microvascular Institute of Integration of Chinese and Western Medicine, Beijing, China
- Yu-Ying Liu
- Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China
- Yu-Ying Liu
- Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Beijing, China
- Yu-Ying Liu
- Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Beijing, China
- Yu-Ying Liu
- State Key Laboratory of Core Technology in Innovative Chinese Medicine, Beijing, China
- Yu-Ying Liu
- Beijing Microvascular Institute of Integration of Chinese and Western Medicine, Beijing, China
- Bai-He Hu
- Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China
- Bai-He Hu
- Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Beijing, China
- Bai-He Hu
- Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Beijing, China
- Bai-He Hu
- State Key Laboratory of Core Technology in Innovative Chinese Medicine, Beijing, China
- Bai-He Hu
- Beijing Microvascular Institute of Integration of Chinese and Western Medicine, Beijing, China
- Chuan-She Wang
- Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing, China
- Chuan-She Wang
- Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China
- Chuan-She Wang
- Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Beijing, China
- Chuan-She Wang
- Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Beijing, China
- Chuan-She Wang
- State Key Laboratory of Core Technology in Innovative Chinese Medicine, Beijing, China
- Chuan-She Wang
- Beijing Microvascular Institute of Integration of Chinese and Western Medicine, Beijing, China
- Jing-Yu Fan
- Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China
- Jing-Yu Fan
- Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Beijing, China
- Jing-Yu Fan
- Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Beijing, China
- Jing-Yu Fan
- State Key Laboratory of Core Technology in Innovative Chinese Medicine, Beijing, China
- Jing-Yu Fan
- Beijing Microvascular Institute of Integration of Chinese and Western Medicine, Beijing, China
- Jing-Yan Han
- Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing, China
- Jing-Yan Han
- Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China
- Jing-Yan Han
- Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Beijing, China
- Jing-Yan Han
- Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Beijing, China
- Jing-Yan Han
- State Key Laboratory of Core Technology in Innovative Chinese Medicine, Beijing, China
- Jing-Yan Han
- Beijing Microvascular Institute of Integration of Chinese and Western Medicine, Beijing, China
- DOI
- https://doi.org/10.3389/fphys.2019.01320
- Journal volume & issue
-
Vol. 10
Abstract
Objective: Lipopolysaccharide (LPS) causes microvascular dysfunction, which is a key episode in the pathogenesis of endotoxemia. This work aimed to investigate the effect of Qing-Ying-Tang (QYT), a compound Chinese medicine in cerebral microcirculation disturbance and brain damage induced by LPS.Methods: Male C57/BL6 mice were continuously transfused with LPS (7.5 mg/kg/h) through the left femoral vein for 2 h. QYT (14.3 g/kg) was given orally 2 h after LPS administration. The dynamics of cerebral microcirculation were evaluated by intravital microscopy. Brain tissue edema was assessed by brain water content and Evans Blue leakage. Cytokines in plasma and brain were evaluated by flow cytometry. Confocal microscopy and Western blot were applied to detect the expression of junction and adhesion proteins, and signaling proteins concerned in mouse brain tissue.Results: Post-treatment with QYT significantly ameliorated LPS-induced leukocyte adhesion to microvascular wall and albumin leakage from cerebral venules and brain tissue edema, attenuated the increase of MCP-1, MIP-1α, IL-1α, IL-6, and VCAM-1 in brain tissue and the activation of NF-κB and expression of MMP-9 in brain. QYT ameliorated the downregulation of claudin-5, occludin, JAM-1, ZO-1, collagen IV as well as the expression and phosphorylation of VE-cadherin in mouse brain.Conclusions: This study demonstrated that QYT protected cerebral microvascular barrier from disruption after LPS by acting on the transcellular pathway mediated by caveolae and paracellular pathway mediated by junction proteins. This result suggests QYT as a potential strategy to deal with endotoxemia.
Keywords
