Determination of anticholinergic effects of p-nitroaniline

Abstract

AbstractCholinesterases allow the nervous system to function properly by catalyzing the breakdown of the neurotransmitter acetylcholine (ACh), a naturally occurring neurotransmitter, into choline and acetic acid. Cholinesterases in the human body are of two types: acetylcholinesterase (AChE; E.C.3.1.1.7) and butyrylcholinesterase (BChE; E.C.3.1.1.8). In this study, the anticholinergic effect of p-Nitroaniline was investigated experimentally and theoretically and compared with tacrine, which is known as an AChE inhibitor. IC50 values were calculated for p-Nitroaniline; 17.77 nM against AChE, 18.73 nM towards BChE. Also, Ki values was found to be 1.80 ± 0.16 nM against AChE and 6.49 ± 1.63 nM on BChE. Docking Score values were calculated as -4.631 for AChE and -3.779 for BChE.

Keywords

• alzheimer hastalığı
• enzim inhibisyonu
• asetilkolinesteraz
• butirilkolinesteraz
• alzheimer’s disease
• enzyme inhibition
• acetylcholinesterase