SELEKSI BAKTERI ASAM LAKTAT PENGHASIL EKSOPOLISAKARIDA

Abstract

Penelitian ini bertujuan untuk mendapatkan bakteri asam laktat penghasil eksopolisakarida (EPS). 37 spesies bakteri asam laktat dari yogurt komersial, kultur indigenous dan Leuconostoc mesenteroiodes FNCC 0023 yang diseleksi kemampuannya untuk menghasilkan eksopolisakarida menggunakan medium (skim 2%, skim2%+sukrosa5%, dan skim2%+sukrosa10%) dan medium agar (whey agar, whey agar+sukrosa5% and whey agar+sukrosa10%) menggunakan metode pour plate and spread plate dan diinkubasi pada suhu 30C. Pada medium cair tidak didapatkan bakteri asam laktat penghasil eksopolisakarida. Leuconostoc mesenteroides FNCC0023 pada whey agar+sukrosa dengan metode pour plate dan spread plate menghasilkan EPS mucoid, pada whey agar dan whey agar+sukrosa dengan metode spread plate menghasilkan EPS ropy. Lactobacillus sp (Dad1) pada whey agar and whey agar+sukrosa produce EPS ropy namun tidak konsisten, dan Lactobacillus reuteri (M11), Lactobacillus plantarum (Mut4), Lactobacillus sake (Mut5) dan Lactobacillus sake (Mut13) pada whey agar dan whey agar+sukrosa menghasilkan EPS mucoid namun tidak konsisten. Disimpulkan bahwa L. mesenteroide FNCC 0023 menghasilkan 2 jenis EPS, mucoid dan ropy. Terdapat beberapa kultur indigenous yang menghasilkan EPS yang tidak konsisten. Kata kunci: Bakteri asam laktat, Leuconostoc mesenteroides FNCC0023, Eksopolisakarida. ABSTRACT The purpose of this study was to find out lactic acid bacteria producing exopolysaccharides. 37 spesies of lactic acid bacteria from commercial yogurt, indigenous culture and Leuconostoc mesenteroiodes FNCC 0023 was selected their ability to produce exopolysaccharides using liquid medium (2% skim, 2% skim+5%sucrose, and 2% skim+10% sucrose) and agar medium (whey agar, whey agar+5%sukrosa and whey agar+10%sukrosa) using pour plate and spread plate which incubated at 30C.The liquid medium not find out lactic acid bacteria producing exopolysaccharides.Leuconostoc mesenteroides FNCC0023 on whey agar+sukrosa with pour plate and spread plate method produce mucoid EPS, on whey agar and whey agar+sukrosa with spread plate method produce ropy EPS. Lactobacillus sp (Dad1) on whey agar and whey agar+sukrosa produce unconsistent ropy EPS, and Lactobacillus reuteri (M11), Lactobacillus plantarum (Mut4), Lactobacillus sake (Mut5) and Lactobacillus sake (Mut13) on whey agar and whey agar+sukrosa produce unconsistent EPS mucoid. We concluded that L. mesenteroides FNCC 0023 produced 2 type of EPS, mucoid and ropy. There were some indogenous culture which unconsistent EPS producers. Keywords: Lactic acid bacteria, Leuconostoc mesenteroides FNCC0023, Exopolysaccharides.