Effect of regulator of ribosome synthesis 1 on proliferation and metastasis abilities of human breast cancer MDA-MB-468 cells

Abstract

Objective To investigate the effect of regulator of ribosome synthesis 1 (RRS1) on the proliferation and metastasis abilities of human breast cancer cells. Methods Western Blot was used to measure the protein expression level of RRS1 in human breast cancer cell lines (MDA-MB-231, MDA-MB-468, BT549, and MCF-7) and normal human breast epithelial cells. MDA-MB-468 cells were infected with sh-RRS1 lentivirus (sh-RRS1 group) and negative-control lentivirus (Con group), and MDA-MB-468 cells without infection were established as Blank group. Lentiviral infection efficiency was observed under a fluorescence microscope for the Con group and the sh-RRS1 group, and quantitative real-time PCR and Western blot were used to measure the mRNA and protein expression levels of RRS1 in cells; CCK-8 assay was used to observe the effect of RRS1 on the viability of MDA-MB-468 cells; scratch assay, Transwell assay, and invasion assay were used to observe the effect of RRS1 on the invasion and migration abilities of MDA-MB-468 cells. Results The expression level of RRS1 in the breast cancer cell lines was significantly higher than that in normal human breast epithelial cells (F=28.71,P<0.05). Compared with the Blank group and the Con group, the sh-RRS1 group had significant reductions in the mRNA and protein expression levels of RRS1 (F=118.10,335.40,P<0.05), cell proliferation activity (F=825.60-2839.00,P<0.05), and the invasion and migration abilities of cells (F=25.60-430.80,P<0.05). Conclusion The RRS1 gene might be involved in the proliferation and metastasis of breast cancer cells, which may be associated with the high expression of RRS1 in cells.

Keywords

• breast neoplasms|cell line, tumor|nuclear proteins|regulator of ribosome synthesis 1|cell proliferation|cell movement