Identification Procedures of Yeast Species Recovered from Portuguese Intensive Care Units

Abstract

Yeast species other than Candida albicans are becoming more clinically relevant in the Intensive Care Unit (ICU) landscape, making it important to identify them correctly. The aim of this study was to evaluate the concordance of the identification of clinical isolates from ICUs, at the species level, by conventional and molecular methods. All isolates (n = 371) underwent identification by cultural, MALDI-TOF MS and PCR. The direct concordance between conventional and molecular identification was 92% (341/371). These results allow us to conclude that culture-based methodologies are still useful to reliably identify the most frequent yeasts, but for rare, uncommon or cryptic species, technologies such as MALDI-TOF MS or PCR are needed.

Keywords

• <i>Candida</i> spp.
• MALDI-TOF MS
• PCR
• yeasts
• identification