Clerodane Diterpenoids from <i>Callicarpa hypoleucophylla</i> and Their Anti-Inflammatory Activity
Yu-Chi Lin,
Jue-Jun Lin,
Shu-Rong Chen,
Tsong-Long Hwang,
Shu-Yen Fang,
Michal Korinek,
Ching-Yeu Chen,
Yun-Sheng Lin,
Tung-Ying Wu,
Ming-Hong Yen,
Chih-Hsin Wang,
Yuan-Bin Cheng
Affiliations
Yu-Chi Lin
Graduate Institute of Natural Products, Center for Natural Product Research and Development, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 80708, Taiwan
Jue-Jun Lin
Graduate Institute of Natural Products, Center for Natural Product Research and Development, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 80708, Taiwan
Shu-Rong Chen
Graduate Institute of Natural Products, Center for Natural Product Research and Development, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 80708, Taiwan
Tsong-Long Hwang
Graduate Institute of Natural Products, Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan
Shu-Yen Fang
Graduate Institute of Natural Products, Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan
Michal Korinek
Graduate Institute of Natural Products, Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan
Ching-Yeu Chen
Department of Physical Therapy, Tzu-Hui Institute of Technology, Pingtung 92641, Taiwan
Yun-Sheng Lin
Department of Biological Science and Technology, Meiho University, Pingtung 912009, Taiwan
Tung-Ying Wu
Department of Biological Science and Technology, Meiho University, Pingtung 912009, Taiwan
Ming-Hong Yen
School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 80708, Taiwan
Chih-Hsin Wang
Division of Pharmacy, Zuoying Branch of Kaohsiung Armed Forces General Hospital, Kaohsiung 81342, Taiwan
Yuan-Bin Cheng
Graduate Institute of Natural Products, Center for Natural Product Research and Development, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 80708, Taiwan
Plants of the genus Callicarpa are known to possess several medicinal effects. The constituents of the Taiwan endemic plant Callicarpa hypoleucophylla have never been studied. Therefore, C. hypoleucophylla was selected for our phytochemical investigation. Two new clerodane-type diterpenoids, named callihypolins A (1) and B (2), along with seven known compounds were isolated from the leaves and twigs of the Lamiaceae plant C. hypoleucophylla and then characterized. The structures of compounds 1 and 2 were elucidated by spectroscopic data analysis, specifically, two-dimension nuclear magnetic resonance (NMR). The anti-inflammatory activity of compounds 1–9 based on the suppression of superoxide anion generation and elastase release was evaluated. Among the isolates, compounds 2–4 showed anti-inflammatory activity (9.52−32.48% inhibition at the concentration 10 μm) by suppressing superoxide anion generation and elastase release. Our findings not only expand the description of the structural diversity of the compounds present in plants of the genus Callicarpa but also highlight the possibility of developing anti-inflammatory agents from Callicarpa endemic species.
