Nature Communications (Mar 2020)
Shotgun ion mobility mass spectrometry sequencing of heparan sulfate saccharides
- Rebecca L. Miller,
- Scott E. Guimond,
- Ralf Schwörer,
- Olga V. Zubkova,
- Peter C. Tyler,
- Yongmei Xu,
- Jian Liu,
- Pradeep Chopra,
- Geert-Jan Boons,
- Márkó Grabarics,
- Christian Manz,
- Johanna Hofmann,
- Niclas G. Karlsson,
- Jeremy E. Turnbull,
- Weston B. Struwe,
- Kevin Pagel
Affiliations
- Rebecca L. Miller
- Copenhagen Center for Glycomics, Department of Cellular & Molecular Medicine, University of Copenhagen
- Scott E. Guimond
- Centre for Glycobiology, Department of Biochemistry, Institute of Integrative Biology, University of Liverpool
- Ralf Schwörer
- Ferrier Research Institute, Victoria University of Wellington, 69 Gracefield Road, Gracefield
- Olga V. Zubkova
- Ferrier Research Institute, Victoria University of Wellington, 69 Gracefield Road, Gracefield
- Peter C. Tyler
- Ferrier Research Institute, Victoria University of Wellington, 69 Gracefield Road, Gracefield
- Yongmei Xu
- Division of Chemical Biology and Medicinal Chemistry, Eshelman School of Pharmacy, University of North Carolina
- Jian Liu
- Division of Chemical Biology and Medicinal Chemistry, Eshelman School of Pharmacy, University of North Carolina
- Pradeep Chopra
- Complex Carbohydrate Research Center, University of Georgia
- Geert-Jan Boons
- Complex Carbohydrate Research Center, University of Georgia
- Márkó Grabarics
- Freie Universitaet Berlin, Institute of Chemistry and Biochemistry
- Christian Manz
- Freie Universitaet Berlin, Institute of Chemistry and Biochemistry
- Johanna Hofmann
- Freie Universitaet Berlin, Institute of Chemistry and Biochemistry
- Niclas G. Karlsson
- Department of Medical Biochemistry and Cell Biology, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg
- Jeremy E. Turnbull
- Copenhagen Center for Glycomics, Department of Cellular & Molecular Medicine, University of Copenhagen
- Weston B. Struwe
- Department of Chemistry, Chemistry Research Laboratory, University of Oxford
- Kevin Pagel
- Freie Universitaet Berlin, Institute of Chemistry and Biochemistry
- DOI
- https://doi.org/10.1038/s41467-020-15284-y
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 12
Abstract
Heparan sulfates (HS) contain functionally relevant structural motifs, but determining their monosaccharide sequence remains challenging. Here, the authors develop an ion mobility mass spectrometry-based method that allows unambiguous characterization of HS sequences and structure-activity relationships.
