Protocol for quantitative analysis of pulsatile contractions and cell extrusion in epithelial tissues of larval zebrafish

Abstract

Summary: Cell elimination by extrusion is important for epithelial homeostasis, but knowing when and where cells will extrude has made in vivo studies difficult. Here, we describe a step-by-step protocol for inducing cell extrusion from the larval zebrafish epidermis. We detail how to capture the dynamics of extrusion via time-lapse imaging and describe how existing protocols can be implemented for the analysis of cell shape changes preceding extrusion events and derivation of mechanical measurements associated with these shape changes.For complete details on the use and execution of this protocol, please refer to Atieh et al. (2021).

Keywords

• cell biology
• developmental biology
• microscopy
• model organisms