Acute exposure to LPS induces cardiac dysfunction via the activation of the NLRP3 inflammasome

Tshiamo T Maluleke; Ashmeetha Manilall; Nandi Shezi; Sooraj Baijnath; Aletta M.E. Millen

doi:10.1038/s41598-024-76066-w

Scientific Reports (Oct 2024)

Acute exposure to LPS induces cardiac dysfunction via the activation of the NLRP3 inflammasome

Tshiamo T Maluleke,
Ashmeetha Manilall,
Nandi Shezi,
Sooraj Baijnath,
Aletta M.E. Millen

Affiliations

Tshiamo T Maluleke: Wits Integrated Molecular Physiology Research Initiative, School of Physiology, Faculty of Health Sciences, Wits Health Consortium (PTY) Ltd, University of The Witwatersrand
Ashmeetha Manilall: Wits Integrated Molecular Physiology Research Initiative, School of Physiology, Faculty of Health Sciences, Wits Health Consortium (PTY) Ltd, University of The Witwatersrand
Nandi Shezi: Wits Integrated Molecular Physiology Research Initiative, School of Physiology, Faculty of Health Sciences, Wits Health Consortium (PTY) Ltd, University of The Witwatersrand
Sooraj Baijnath: Wits Integrated Molecular Physiology Research Initiative, School of Physiology, Faculty of Health Sciences, Wits Health Consortium (PTY) Ltd, University of The Witwatersrand
Aletta M.E. Millen: Wits Integrated Molecular Physiology Research Initiative, School of Physiology, Faculty of Health Sciences, Wits Health Consortium (PTY) Ltd, University of The Witwatersrand

DOI: https://doi.org/10.1038/s41598-024-76066-w
Journal volume & issue: Vol. 14, no. 1
pp. 1 – 14

Abstract

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Abstract Systemic inflammation contributes to left ventricular (LV) dysfunction, however the role of the NLRP3 inflammasome in LV dysfunction in acute inflammatory conditions is unclear. This study investigated the role of the NLRP3 inflammasome in acute (24 h) cardiac structural and functional changes in vivo and in vitro in lipopolysaccharide (LPS)-induced inflammation. LPS-treated Sprague-Dawley (SD) rats showed increased LPS metabolite abundance in their LVs as measured by atmospheric pressure matrix-assisted laser desorption ionisation (AP-MALDI) mass spectrometry imaging (MSI). Echocardiography and histology showed that in LPS-exposed rats, LV internal diameter was decreased, with evidence of macrophage infiltration and oedema. However, there were no changes in LV wall thickness or collagen volume. Additionally, LPS-exposed rats exhibited impaired LV relaxation, potentially contributing to decreased stroke volume. While global systolic function was preserved, LPS exposure in SD rats resulted in impaired myocardial deformation assessed by speckle-tracking echocardiography. Exposure to LPS resulted in upregulation of the expression of components of the NLRP3 inflammasome in rodents. In vitro LPS exposure resulted in increased gene expression of NLRP3 and downstream cytokines IL-1β and IL-18, antioxidant SOD2, and elevated markers of pyroptosis (GSDMD) which were inhibited by treatment with a NLRP3 antagonist. However, LPS-induced increases in the gene expression of apoptosic markers (BAX/Bcl2) were not impacted by NLRP3 antagonism. These findings suggest that inflammation induced adverse cardiac structural and functional changes is, at least in part, mediated by the NLRP3 inflammasome in acute, high-grade inflammatory states. In addition, in vitro findings suggest that while the NLRP3 inflammasome mediates pyroptotic pathways, regulation of apoptosis that is independent of the inflammasome.

Published in Scientific Reports

ISSN: 2045-2322 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Medicine; Science
Website: https://www.nature.com/srep/

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