4D analysis of malaria parasite invasion offers insights into erythrocyte membrane remodeling and parasitophorous vacuole formation

Niall D. Geoghegan; Cindy Evelyn; Lachlan W. Whitehead; Michal Pasternak; Phoebe McDonald; Tony Triglia; Danushka S. Marapana; Daryan Kempe; Jennifer K. Thompson; Michael J. Mlodzianoski; Julie Healer; Maté Biro; Alan F. Cowman; Kelly L. Rogers

doi:10.1038/s41467-021-23626-7

Nature Communications (Jun 2021)

4D analysis of malaria parasite invasion offers insights into erythrocyte membrane remodeling and parasitophorous vacuole formation

Niall D. Geoghegan,
Cindy Evelyn,
Lachlan W. Whitehead,
Michal Pasternak,
Phoebe McDonald,
Tony Triglia,
Danushka S. Marapana,
Daryan Kempe,
Jennifer K. Thompson,
Michael J. Mlodzianoski,
Julie Healer,
Maté Biro,
Alan F. Cowman,
Kelly L. Rogers

Affiliations

Niall D. Geoghegan: The Walter & Eliza Hall Institute of Medical Research
Cindy Evelyn: The Walter & Eliza Hall Institute of Medical Research
Lachlan W. Whitehead: The Walter & Eliza Hall Institute of Medical Research
Michal Pasternak: The Walter & Eliza Hall Institute of Medical Research
Phoebe McDonald: The Walter & Eliza Hall Institute of Medical Research
Tony Triglia: The Walter & Eliza Hall Institute of Medical Research
Danushka S. Marapana: The Walter & Eliza Hall Institute of Medical Research
Daryan Kempe: EMBL Australia, Single Molecule Science Node, School of Medical Sciences, University of New South Wales
Jennifer K. Thompson: The Walter & Eliza Hall Institute of Medical Research
Michael J. Mlodzianoski: The Walter & Eliza Hall Institute of Medical Research
Julie Healer: The Walter & Eliza Hall Institute of Medical Research
Maté Biro: EMBL Australia, Single Molecule Science Node, School of Medical Sciences, University of New South Wales
Alan F. Cowman: The Walter & Eliza Hall Institute of Medical Research
Kelly L. Rogers: The Walter & Eliza Hall Institute of Medical Research

DOI: https://doi.org/10.1038/s41467-021-23626-7
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 16

Abstract

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Here, Geoghegan, Evelyn et al. provide a lattice light-sheet microscopy based 4D imaging pipeline to quantitatively investigate Plasmodium spp. invasion and show that the nascent parasitophorous vacuole is predominantly formed from host’s erythrocyte membrane and undergoes continuous remodeling throughout invasion.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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