Morphological features of bone tissue regenerates in experimental animals when using osteotropic drugs for recovery bone defects

Abstract

The aim of the study was to determine the effectiveness using of osteotropic drugs for the restoration of bone defects. Materials and methods. The model experiment was performing on male Wistar rats. The animals has been simulated a bone defect into which was implanted:"mp3 Osteobiol" (1st experimental group - 14 animals), PRP coated with fibrin glue (2nd experimental group - 13 animals) and PRP + "mp3 Osteobiol" (3rd experimental group - 15 animals). The histological material were staining with hematoxylin-eosin (according to Van – Gizon). Microscopic studies were performing on a Leica MDE optical microscope at a magnification of x 100, x 200. Results. At the 60th day of observation, after implantation of "mp3 OsteoBiol" + PRP, a pronounced growth of fibrous, loose, not quite mature connective tissue was visualized, with the proliferation of giant multinucleated cells, macrophages, osteoblasts and the formation of osteoids. At the end of the experiment (90th day) in the bone regenerates of animals of group III noted the active formation of the newly formed bone lamellar structure, the formation of osteons, the bone marrow canal was filled with bone marrow. Conclusion. According to histological studies, it was found that our proposed composition, containing "mp3 OsteoBiol" and PRP, accelerated the process of bone regeneration, compared with the groups where the above agents were used alone, without inflammation, with the formation of bone, which has mature in nature.

Keywords

• bone tissue
• osteotropic drugs
• PRP
• osteoinduction