MONOCYTES SUBPOPULATIONS AND CHEMILUMINESCENT ACTIVITY IN PATIENTS WITH RENAL CELL CARCINOMA

Abstract

The aim of present study was to investigate the relationship between phenotypic features of monocytes and intensity of “respiratory burst” in the patients with renal cell carcinoma (RCC). A total of 73 patients with RCC (Т3N0М0, clear cell type) participated in the study. Phenotyping of blood monocytes was performed by flow cytometry. The level of “respiratory burst” in monocytes was determined using spontaneous and zymosan-induced luminol- and lucigenin-dependent chemiluminescence. Suffficient changes in phenotypic structure and intensity of “respiratory burst” were identified in peripheral blood monocytes of the patients. Alterations of monocytic subpopulations in RCC were characterized by increased numbers of cells with the CD14lowCD16+ (“non-classical”) phenotype. The imbalance in expression of activation markers was found among monocyte populations from cancer patients; we have revealed a reduced number of monocytes expressing HLA-DR-antigen, and increased number of CD64-positive cells. Meanwhile, intensity of “respiratory burst” in the total monocyte population proved to be reduced in RCC patients. In this case, the characteristic features of the “respiratory burst” intensity distribution among monocytes were as follows: in RCC, a reduced “respiratory burst” activity was found in monocytes with CD14+CD16- phenotype, being, however, increased in the monocytes with CD14+CD16+ and CD14lowCD16+ phenotypes. Such redistribution may be due to increasing role of the given monocyte subsets in immunopathogenesis of renal cell carcinoma.

Keywords

• renal cancer cells
• monocytes
• subpopulations
• functional activity
• activation markers
• respiratory burst