Small molecule inhibition of ATM kinase increases CRISPR-Cas9 1-bp insertion frequency

Heysol C. Bermudez-Cabrera; Sannie Culbertson; Sammy Barkal; Benjamin Holmes; Max W. Shen; Sophia Zhang; David K. Gifford; Richard I. Sherwood

doi:10.1038/s41467-021-25415-8

Nature Communications (Aug 2021)

Small molecule inhibition of ATM kinase increases CRISPR-Cas9 1-bp insertion frequency

Heysol C. Bermudez-Cabrera,
Sannie Culbertson,
Sammy Barkal,
Benjamin Holmes,
Max W. Shen,
Sophia Zhang,
David K. Gifford,
Richard I. Sherwood

Affiliations

Heysol C. Bermudez-Cabrera: Division of Genetics, Department of Medicine, Brigham and Women’s Hospital and Harvard Medical School
Sannie Culbertson: Division of Genetics, Department of Medicine, Brigham and Women’s Hospital and Harvard Medical School
Sammy Barkal: Division of Genetics, Department of Medicine, Brigham and Women’s Hospital and Harvard Medical School
Benjamin Holmes: Computer Science and Artificial Intelligence Laboratory, Massachusetts Institute of Technology
Max W. Shen: Computational and Systems Biology Program, Massachusetts Institute of Technology
Sophia Zhang: Division of Genetics, Department of Medicine, Brigham and Women’s Hospital and Harvard Medical School
David K. Gifford: Computer Science and Artificial Intelligence Laboratory, Massachusetts Institute of Technology
Richard I. Sherwood: Division of Genetics, Department of Medicine, Brigham and Women’s Hospital and Harvard Medical School

DOI: https://doi.org/10.1038/s41467-021-25415-8
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 10

Abstract

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The mutational outcome of CRISPR-Cas9 editing can be both predictable and targeted. Here the authors show that ATM inhibitor KU-60019 increases 1 bp insertions at the targeted locus.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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