Dynamic light scattering - a simple and sensitive method of determination immune complexes in biological liquids

Abstract

In this paper we introduce new approach in formed in heterogeneous biological fluids immune complexes analysis. The approach is based on an analysis of the contribution of immune complexes in dynamic light scattering, recorded by laser correlometer with heterodyne measurement circuit. We demonstrated our approach sensitivity using embryonic calf serum model in addition of monoclonal antibody against hepatocuprein. Proposed approach is capable to detect forming immune complexes by adding antigen at a concentration greater than 150 pg/ml. Using immunoaffinity techniques we confirmed the lawfulness of identified peaks in the histograms of dynamic light scattering as appropriate immune complexes. For these purposes, we selectively removed immune complexes using staphylococcal protein A sepharose immobilized on the particles or increased in size immune complexes due to their further aggregation using antibodies specific for human immunoglobulin. The proposed approach can detect immune complexes in various biological fluids.

Keywords

• immune complexes
• blood plasma
• diagnostic method
• dynamic light scattering
• иммунные комплексы
• плазма крови
• диагностический метод
• динамическое светорассеяние