Numerical focusing and the field of view in interference microscopy

Abstract

This paper presents an analysis of the effects occurring at the borders of the field of view in numerically focused imaging of optically defocused objects in interference microscopy systems with spatially coherent illumination of an object. Equations describing the borders of regions of different types in numerically focused images with respect to the defocus parameters are obtained. Equations for estimating the acceptable limits of defocus in numerically focused imaging are obtained. Experimental investigation of the effects, occurring at the borders of the field of view in numerically focused imaging of optically defocused objects in a digital holographic microscope with illumination in transmission is performed.

Keywords

• numerical focusing
• interference microscopy
• digital holographic microscopy
• optical coherence microscopy