Frontiers in Veterinary Science (Dec 2020)
Phosphoproteomics to Characterize Host Response During H3N2 Canine Influenza Virus Infection of Dog Lung
- Yongbo Liu,
- Yongbo Liu,
- Yongbo Liu,
- Cheng Fu,
- Shaotang Ye,
- Shaotang Ye,
- Shaotang Ye,
- Yingxin Liang,
- Yingxin Liang,
- Yingxin Liang,
- Zhonghe Qi,
- Zhonghe Qi,
- Zhonghe Qi,
- Congwen Yao,
- Congwen Yao,
- Congwen Yao,
- Zhen Wang,
- Zhen Wang,
- Zhen Wang,
- Ji Wang,
- Ji Wang,
- Ji Wang,
- Siqi Cai,
- Siqi Cai,
- Siqi Cai,
- Shiyu Tang,
- Shiyu Tang,
- Shiyu Tang,
- Ying Chen,
- Ying Chen,
- Ying Chen,
- Shoujun Li,
- Shoujun Li,
- Shoujun Li
Affiliations
- Yongbo Liu
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Yongbo Liu
- Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, China
- Yongbo Liu
- Guangdong Technological Engineering Research Center for Pet, Guangzhou, China
- Cheng Fu
- College of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, China
- Shaotang Ye
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Shaotang Ye
- Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, China
- Shaotang Ye
- Guangdong Technological Engineering Research Center for Pet, Guangzhou, China
- Yingxin Liang
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Yingxin Liang
- Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, China
- Yingxin Liang
- Guangdong Technological Engineering Research Center for Pet, Guangzhou, China
- Zhonghe Qi
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Zhonghe Qi
- Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, China
- Zhonghe Qi
- Guangdong Technological Engineering Research Center for Pet, Guangzhou, China
- Congwen Yao
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Congwen Yao
- Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, China
- Congwen Yao
- Guangdong Technological Engineering Research Center for Pet, Guangzhou, China
- Zhen Wang
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Zhen Wang
- Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, China
- Zhen Wang
- Guangdong Technological Engineering Research Center for Pet, Guangzhou, China
- Ji Wang
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Ji Wang
- Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, China
- Ji Wang
- Guangdong Technological Engineering Research Center for Pet, Guangzhou, China
- Siqi Cai
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Siqi Cai
- Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, China
- Siqi Cai
- Guangdong Technological Engineering Research Center for Pet, Guangzhou, China
- Shiyu Tang
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Shiyu Tang
- Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, China
- Shiyu Tang
- Guangdong Technological Engineering Research Center for Pet, Guangzhou, China
- Ying Chen
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Ying Chen
- Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, China
- Ying Chen
- Guangdong Technological Engineering Research Center for Pet, Guangzhou, China
- Shoujun Li
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Shoujun Li
- Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, China
- Shoujun Li
- Guangdong Technological Engineering Research Center for Pet, Guangzhou, China
- DOI
- https://doi.org/10.3389/fvets.2020.585071
- Journal volume & issue
-
Vol. 7
Abstract
Avian-origin H3N2 canine influenza viruses (CIVs) cause severe contagious respiratory disease in dogs, and quickly adapt to new environments. To further understand the mechanism of virus infection and host-virus interactions, we characterized the complete phosphoproteome of dogs infected with H3N2 CIV. Nine-week-old Beagle dogs were inoculated intranasally with 106 EID50 of A/canine/Guangdong/04/2014 (H3N2) virus. Lung sections were harvested at 5 days post-inoculation (dpi) and processed for global and quantitative analysis of differentially expressed phosphoproteins. A total of 1,235 differentially expressed phosphorylated proteins were identified in the dog lung after H3N2 CIV infection, and 3,016 modification sites were identified among all differentially expressed proteins. We then performed an enrichment analysis of functional annotations using Kyoto Encyclopedia of Genes and Genomes (KEGG) and gene ontology (GO) database analyses to predict the functions of the identified differential phosphoproteins. Our data indicate that H3N2 CIV infection causes dramatic changes in the host protein phosphorylation of dog lungs. To our knowledge, this is the first study to assess the effect of H3N2 CIV infection on the phosphoproteome of beagles. These data provide novel insights into H3N2-CIV-triggered regulatory phosphorylation circuits and signaling networks and may improve our understanding of the mechanisms underlying CIV pathogenesis in dogs.
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