Transcriptome analysis reveals the molecular mechanism of differences in growth between photoautotrophy and heterotrophy in Chlamydomonas reinhardtii

Jing Chen; Jing Chen; Jing Chen; Yuanhao Chen; Yuanhao Chen; Yuanhao Chen; Weiling He; Weiling He; Weiling He; Honghao Liang; Honghao Liang; Honghao Liang; Ting Hong; Ting Hong; Ting Hong; Tangcheng Li; Tangcheng Li; Tangcheng Li; Hong Du; Hong Du; Hong Du

doi:10.3389/fpls.2024.1407915

Frontiers in Plant Science (Jun 2024)

Transcriptome analysis reveals the molecular mechanism of differences in growth between photoautotrophy and heterotrophy in Chlamydomonas reinhardtii

Jing Chen,
Jing Chen,
Jing Chen,
Yuanhao Chen,
Yuanhao Chen,
Yuanhao Chen,
Weiling He,
Weiling He,
Weiling He,
Honghao Liang,
Honghao Liang,
Honghao Liang,
Ting Hong,
Ting Hong,
Ting Hong,
Tangcheng Li,
Tangcheng Li,
Tangcheng Li,
Hong Du,
Hong Du,
Hong Du

Affiliations

Jing Chen: Guangdong Provincial Key Laboratory of Marine Biotechnology, STU-UNIVPM Joint Algal Research Center, Institute of Marine Sciences, Shantou University, Shantou, Guangdong, China
Jing Chen: Southern Marine Science and Engineering Guangdong Laboratory, Guangzhou, Guangdong, China
Jing Chen: Guangdong Provincial Key Laboratory of Marine Disaster Prediction and Prevention, Shantou University, Shantou, Guangdong, China
Yuanhao Chen: Guangdong Provincial Key Laboratory of Marine Biotechnology, STU-UNIVPM Joint Algal Research Center, Institute of Marine Sciences, Shantou University, Shantou, Guangdong, China
Yuanhao Chen: Southern Marine Science and Engineering Guangdong Laboratory, Guangzhou, Guangdong, China
Yuanhao Chen: Guangdong Provincial Key Laboratory of Marine Disaster Prediction and Prevention, Shantou University, Shantou, Guangdong, China
Weiling He: Guangdong Provincial Key Laboratory of Marine Biotechnology, STU-UNIVPM Joint Algal Research Center, Institute of Marine Sciences, Shantou University, Shantou, Guangdong, China
Weiling He: Southern Marine Science and Engineering Guangdong Laboratory, Guangzhou, Guangdong, China
Weiling He: Guangdong Provincial Key Laboratory of Marine Disaster Prediction and Prevention, Shantou University, Shantou, Guangdong, China
Honghao Liang: Guangdong Provincial Key Laboratory of Marine Biotechnology, STU-UNIVPM Joint Algal Research Center, Institute of Marine Sciences, Shantou University, Shantou, Guangdong, China
Honghao Liang: Southern Marine Science and Engineering Guangdong Laboratory, Guangzhou, Guangdong, China
Honghao Liang: Guangdong Provincial Key Laboratory of Marine Disaster Prediction and Prevention, Shantou University, Shantou, Guangdong, China
Ting Hong: Guangdong Provincial Key Laboratory of Marine Biotechnology, STU-UNIVPM Joint Algal Research Center, Institute of Marine Sciences, Shantou University, Shantou, Guangdong, China
Ting Hong: Southern Marine Science and Engineering Guangdong Laboratory, Guangzhou, Guangdong, China
Ting Hong: Guangdong Provincial Key Laboratory of Marine Disaster Prediction and Prevention, Shantou University, Shantou, Guangdong, China
Tangcheng Li: Guangdong Provincial Key Laboratory of Marine Biotechnology, STU-UNIVPM Joint Algal Research Center, Institute of Marine Sciences, Shantou University, Shantou, Guangdong, China
Tangcheng Li: Southern Marine Science and Engineering Guangdong Laboratory, Guangzhou, Guangdong, China
Tangcheng Li: Guangdong Provincial Key Laboratory of Marine Disaster Prediction and Prevention, Shantou University, Shantou, Guangdong, China
Hong Du: Guangdong Provincial Key Laboratory of Marine Biotechnology, STU-UNIVPM Joint Algal Research Center, Institute of Marine Sciences, Shantou University, Shantou, Guangdong, China
Hong Du: Southern Marine Science and Engineering Guangdong Laboratory, Guangzhou, Guangdong, China
Hong Du: Guangdong Provincial Key Laboratory of Marine Disaster Prediction and Prevention, Shantou University, Shantou, Guangdong, China

DOI: https://doi.org/10.3389/fpls.2024.1407915
Journal volume & issue: Vol. 15

Abstract

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BackgroundThe green alga Chlamydomonas reinhardtii can grow photoautotrophically utilizing light and CO2, and heterotrophically utilizing acetate. The physiological and biochemical responses of autotrophy and heterotrophy are different in C. reinhardtii. However, there is no complete understanding of the molecular physiology between autotrophy and heterotrophy. Therefore, we performed biochemical, molecular and transcriptome analysis of C. reinhardtii between autotrophy and heterotrophy.ResultsThe cell growth characterization demonstrated that heterotrophic cell had enhanced growth rates, and autotrophic cell accumulated more chlorophyll. The transcriptome data showed that a total of 2,970 differentially expressed genes (DEGs) were identified from photoautotrophy 12h (P12h) to heterotrophy 12h (H12h). The DEGs were involved in photosynthesis, the tricarboxylic acid cycle (TCA), pyruvate and oxidative phosphorylation metabolisms. Moreover, the results of qRT-PCR revealed that the relative expression levels of malate dehydrogenase (MDH), succinate dehydrogenase (SDH), ATP synthase (ATPase), and starch synthase (SSS) were increased significantly from P12h and H12h. The protein activity of NAD-malate dehydrogenase (NAD-MDH) and succinate dehydrogenase (SDH) were significantly higher in the H12h group.ConclusionThe above results indicated that the high growth rate observed in heterotrophic cell may be the effects of environmental or genetic regulation of photosynthesis. Therefore, the identification of novel candidate genes in heterotrophy will contribute to the development of microalga strains with higher growth capacity and better performance for biomass production.

Published in Frontiers in Plant Science

ISSN: 1664-462X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Agriculture: Plant culture
Website: https://www.frontiersin.org/journals/plant-science

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