A simple and versatile design concept for fluorophore derivatives with intramolecular photostabilization

Jasper H. M. van der Velde; Jens Oelerich; Jingyi Huang; Jochem H. Smit; Atieh Aminian Jazi; Silvia Galiani; Kirill Kolmakov; Giorgos Gouridis; Christian Eggeling; Andreas Herrmann; Gerard Roelfes; Thorben Cordes

doi:10.1038/ncomms10144

Nature Communications (Jan 2016)

A simple and versatile design concept for fluorophore derivatives with intramolecular photostabilization

Jasper H. M. van der Velde,
Jens Oelerich,
Jingyi Huang,
Jochem H. Smit,
Atieh Aminian Jazi,
Silvia Galiani,
Kirill Kolmakov,
Giorgos Gouridis,
Christian Eggeling,
Andreas Herrmann,
Gerard Roelfes,
Thorben Cordes

Affiliations

Jasper H. M. van der Velde: Molecular Microscopy Research Group, Zernike Institute for Advanced Materials, University of Groningen
Jens Oelerich: Stratingh Institute for Chemistry, University of Groningen
Jingyi Huang: Department of Polymer Chemistry, Zernike Institute for Advanced Materials, University of Groningen
Jochem H. Smit: Molecular Microscopy Research Group, Zernike Institute for Advanced Materials, University of Groningen
Atieh Aminian Jazi: Molecular Microscopy Research Group, Zernike Institute for Advanced Materials, University of Groningen
Silvia Galiani: MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford
Kirill Kolmakov: Department NanoBiophotonics, Max-Planck-Institute of Molecular Medicine
Giorgos Gouridis: Molecular Microscopy Research Group, Zernike Institute for Advanced Materials, University of Groningen
Christian Eggeling: MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford
Andreas Herrmann: Department of Polymer Chemistry, Zernike Institute for Advanced Materials, University of Groningen
Gerard Roelfes: Stratingh Institute for Chemistry, University of Groningen
Thorben Cordes: Molecular Microscopy Research Group, Zernike Institute for Advanced Materials, University of Groningen

DOI: https://doi.org/10.1038/ncomms10144
Journal volume & issue: Vol. 7, no. 1
pp. 1 – 17

Abstract

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Synthetic organic fluorophores are powerful tools for bioimaging, but frequently display shortened observation times and signal fluctuations. Here, the authors report a general method to covalently label a biomolecule with a fluorophore and photostabilizer, reducing unwanted photophysical effects by intramolecular quenching of reactive fluorophore states.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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