Neoplasia: An International Journal for Oncology Research (Feb 2025)
Comparison of differences in transcriptional and genetic profiles between intra-central nervous system and extra-central nervous system large B-cell lymphoma
- Shu Wang,
- Hong Chen,
- Bo Dai,
- Kang Zheng,
- Jiajun Zheng,
- Yuqi Zhu,
- Yan Yuan,
- Tianling Ding,
- Qian Wang,
- Liqian Xie,
- Rui Feng,
- Fengping Zhu,
- Jianbin Xiang,
- Weiqun Ding,
- Hong Ding,
- Yuan Li,
- Xiaodong Gu,
- Kunpeng Wu,
- Yifan Yuan,
- Jianping Song,
- Dongxiao Zhuang,
- Haoshu Zhong,
- Hanfeng Wu,
- Ying Mao,
- Tong Chen
Affiliations
- Shu Wang
- Department of Hematology, Huashan Hospital, Fudan University, Shanghai 200040, PR China
- Hong Chen
- Department of Pathology, Huashan Hospital, Fudan University, Shanghai 200040, PR China
- Bo Dai
- Department of Hematology, Huashan Hospital, Fudan University, Shanghai 200040, PR China
- Kang Zheng
- Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai 200040, PR China
- Jiajun Zheng
- Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai 200040, PR China
- Yuqi Zhu
- Department of Radiology, Huashan Hospital, Fudan University, Shanghai 200040, PR China
- Yan Yuan
- Department of Hematology, Huashan Hospital, Fudan University, Shanghai 200040, PR China
- Tianling Ding
- Department of Hematology, Huashan Hospital, Fudan University, Shanghai 200040, PR China
- Qian Wang
- Department of Hematology, Huashan Hospital, Fudan University, Shanghai 200040, PR China
- Liqian Xie
- Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai 200040, PR China
- Rui Feng
- Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai 200040, PR China; National Center for Neurological Disorders, Huashan Hospital, Fudan University, Shanghai 200040, PR China; Neurosurgical Institute of Fudan University, Huashan Hospital, Shanghai 200040, PR China
- Fengping Zhu
- Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai 200040, PR China
- Jianbin Xiang
- Department of General Surgery, Huashan Hospital, Fudan University, Shanghai 200040, PR China
- Weiqun Ding
- Department of Gastroenterology, Huashan Hospital, Fudan University, Shanghai 200040, PR China
- Hong Ding
- Department of Ultrasound, Huashan Hospital, Fudan University, Shanghai 200040, PR China
- Yuan Li
- Department of Radiology, Huashan Hospital, Fudan University, Shanghai 200040, PR China
- Xiaodong Gu
- Department of Gastrointestinal Surgery, Huashan Hospital, Fudan University, Shanghai 200040, PR China
- Kunpeng Wu
- Department of Hematology, Huashan Hospital, Fudan University, Shanghai 200040, PR China
- Yifan Yuan
- Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai 200040, PR China; National Center for Neurological Disorders, Huashan Hospital, Fudan University, Shanghai 200040, PR China
- Jianping Song
- Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai 200040, PR China; National Center for Neurological Disorders, Huashan Hospital, Fudan University, Shanghai 200040, PR China; Neurosurgical Institute of Fudan University, Huashan Hospital, Shanghai 200040, PR China; Shanghai Clinical Medical Center of Neurosurgery, Shanghai 200040, PR China; Shanghai Key Laboratory of Brain Function Restoration and Neural Regeneration, Shanghai 200040, PR China
- Dongxiao Zhuang
- Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai 200040, PR China
- Haoshu Zhong
- Department of Hematology, Huashan Hospital, Fudan University, Shanghai 200040, PR China
- Hanfeng Wu
- Department of Neurosurgery, Shanghai Gamma Hospital, Shanghai 200235, PR China; Corresponding author: Department of Neurosurgery, Shanghai Gamma Hospital, 518 Wuzhong East Road, Shanghai 200235, PR China.
- Ying Mao
- Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai 200040, PR China; Corresponding author: Department of Neurosurgery, Huashan Hospital, Fudan University. 12 Wulumuqi Middle Road, Shanghai 200040, PR China.
- Tong Chen
- Department of Hematology, Huashan Hospital, Fudan University, Shanghai 200040, PR China; Corresponding author: Department of Hematology, Huashan Hospital, Fudan University. 12 Wulumuqi Middle Road, Shanghai 200040, PR China.
- Journal volume & issue
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Vol. 60
p. 101119
Abstract
Primary central nervous system diffused large B-cell lymphoma (PCNS-DLBCL) is a rare type of non-Hodgkin lymphoma restricted to the central nervous system (CNS). To explore its specific pathogenesis and therapeutic targets, we performed multi-omics sequencing on tumor samples from patients diagnosed with PCNS-DLBCL, secondary CNS-DLBCL or extracranial (ec) DLBCL.By single-cell RNA sequencing, highly proliferated and dark zone (DZ)-related B cell subclusters, MKI67_B1, PTTG1_B2 and BTG1_B3, were predominant significantly in PCNS-DLBCL. Compared to SCNS-DLBCL and ecDLBCL, an immune-suppressive tumor microenvironment was observed in PCNS-DLBCL by analysis of immune-stimulating/inhibitory ligand‒receptor (L-R) pairs. By performing whole-exome sequencing in 93 patients, mutations enriched in BCR-NFkB and TLR pathways and the cooperation of these two pathways were found to be predominant in PCNS-DLBCL comparing to nonGCB-ecDLBCL. In summary, our study provides comprehensive insights into the transcriptomic and genetic characteristics of PCNS-DLBCL in contrast to ecDLBCL and will help dissect the oncogenic mechanism of this disease.
