Bio-Protocol (Jun 2018)

Increasing the Membrane Permeability of a Fern with DMSO

  • Marcelo Garcés

DOI
https://doi.org/10.21769/BioProtoc.2896
Journal volume & issue
Vol. 8, no. 12

Abstract

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Cell membrane prevents the entrance of extra molecules (e.g., transcription and translation inhibitors) into the cell. For studying the physiological effects of transcription and translation inhibitors on Hymenophyllum caudiculatum fronds, we incubate fronds with 0.1% DMSO to test if this increases cell membrane permeability relative to incubation with ultrapure water. The study showed that DMSO could significantly improve the cell membrane permeability of filmy fronds.