Production of Lipolytic Enzymes by Xylotrophic Basidiomycetes

Abstract

Twenty strains of basidiomycetes were screened for lipolytic activity. Two strains - Trametes versicolor 1 and Hericium erinaceus 0912 - were able to produce lipolytic enzymes during solid medium and submerged cultivation. Oleic acid and yeast extract were selected as the best carbon and nitrogen sources for Trametes versicolor 1, oleic acid, soybean meal and corn steep liquor - for Hericium erinaceus 0912. Hericium erinaceus 0912 accumulated the maximum amount of lipolytic enzymes in the culture medium after 120 h, Trametes versicolor 1 - after 36 h. The culture medium proteins were precipitated by ammonium sulfate and dialyzed, after which the enzyme preparations of lipases were isolated by gel filtration. The weight of the preparation of lipases from H.erinaceus 0912 was 5.5 times greater than the weight of the preparation of lipases from T.versicolor 1, however the activity of the lipases from T.versicolor 1(792.6 mU/mg protein) was three times higher than that of the lipases from H.erinaceus 0912 (204.55 mU/mg protein).

Keywords

• basidiomycetes strains
• lipolytic enzymes
• activity