Bioceramic root canal sealers have been introduced in clinical dental use, but less is known about the antibacterial activity against Streptococcus mutans, Streptococcus salivarius, and Streptococcus sanguis. The purpose of the study is to compare new bioceramic sealers with a traditional zinc-oxide eugenol material considered as a control. The different bioceramic root canal sealants tested were FillRoot ST, BioRoot™RCS, Well-Root™ PT, and CeraSeal. In vitro antibacterial activity against Streptococci was assessed using the agar disc diffusion test at two different intervals, 24 h and 48 h. A non-parametric statistical analysis was performed to compare the inhibition zones for each of the different materials. Bioceramic root canal sealers showed mild antibacterial activity, while zinc-oxide eugenol-based material showed a stronger inhibition of Streptococci diffusion. No differences were detected for the measurements of inhibition zones between 24 h and 48 h except for FillRoot ST and BioRoot™RCS.
