Preparation of Local Diagnostic Kit for Salmonella typhimurium

Abstract

Some of somatic and flageller antgens from both the human and animal isolates were prepared, and were used to Immunize a group of rabbits by intravenous injections at an upgrading doses.The results of this immunization showed the gaining of an excellent hyperimmune serum. With the use of absorptions technique amonospecific antiserum was obtained for the specific injected antigenes, the standardization of the antibodies showed a highly immune response reading. 1: 2560 , 1: 5120 to H:I and O: 4.5 Antigens respectively for both antisera. This study showed the report for the 1st time the preparation for diagnostic kit for somatic and flagellar antigens to S. typhimurium comparing this method to Widal test with a good results and Response