Química Nova (Jan 2010)

Validação e estimativa da incerteza de método para análise de licopeno e β-caroteno em polpa de tomate por cromatografia líquida de alta eficiência

  • Adriana Barreto Alves,
  • Marta Gomes da Silva,
  • Paulo Roberto Nogueira Carvalho,
  • Lizziane Cynara Vissotto,
  • Neura Bragagnolo

DOI
https://doi.org/10.1590/S0100-40422010000900025
Journal volume & issue
Vol. 33, no. 9
pp. 1962 – 1966

Abstract

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A method to quantify lycopene and β-carotene in freeze dried tomato pulp by high performance liquid chromatography (HLPC) was validated according to the criteria of selectivity, sensitivity, precision and accuracy, and uncertainty estimation of measurement was determined with data obtained in the validation. The validated method presented is selective in terms of analysis, and it had a good precision and accuracy. Detection limit for lycopene and β-carotene was 4.2 and 0.23 mg 100 g-1, respectively. The estimation of expanded uncertainty (K = 2) for lycopene was 104 ± 21 mg 100 g-1 and for β-carotene was 6.4 ± 1.5 mg 100 g-1.

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