Frontiers in Immunology (Mar 2020)
Phagocyte Transcriptomic Analysis Reveals Focal Adhesion Kinase (FAK) and Heparan Sulfate Proteoglycans (HSPGs) as Major Regulators in Anti-bacterial Defense of Crassostrea hongkongensis
- Yue Lin,
- Yue Lin,
- Fan Mao,
- Fan Mao,
- Fan Mao,
- Nai-Kei Wong,
- Xiangyu Zhang,
- Xiangyu Zhang,
- Kunna Liu,
- Kunna Liu,
- Minwei Huang,
- Minwei Huang,
- Minwei Huang,
- Haitao Ma,
- Haitao Ma,
- Haitao Ma,
- Zhiming Xiang,
- Zhiming Xiang,
- Zhiming Xiang,
- Jun Li,
- Jun Li,
- Jun Li,
- Shu Xiao,
- Shu Xiao,
- Shu Xiao,
- Yang Zhang,
- Yang Zhang,
- Yang Zhang,
- Ziniu Yu,
- Ziniu Yu,
- Ziniu Yu
Affiliations
- Yue Lin
- CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Science, Guangzhou, China
- Yue Lin
- University of Chinese Academy of Sciences, Beijing, China
- Fan Mao
- CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Science, Guangzhou, China
- Fan Mao
- Innovation Academy of South China Sea Ecology and Environmental Engineering (ISEE), Chinese Academy of Sciences, Guangzhou, China
- Fan Mao
- Southern Marine Science and Engineering Guangdong Laboratory, Guangzhou, China
- Nai-Kei Wong
- National Clinical Research Center for Infectious Diseases, Shenzhen Third People's Hospital, The Second Hospital Affiliated to Southern University of Science and Technology, Shenzhen, China
- Xiangyu Zhang
- CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Science, Guangzhou, China
- Xiangyu Zhang
- University of Chinese Academy of Sciences, Beijing, China
- Kunna Liu
- CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Science, Guangzhou, China
- Kunna Liu
- University of Chinese Academy of Sciences, Beijing, China
- Minwei Huang
- CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Science, Guangzhou, China
- Minwei Huang
- Innovation Academy of South China Sea Ecology and Environmental Engineering (ISEE), Chinese Academy of Sciences, Guangzhou, China
- Minwei Huang
- Southern Marine Science and Engineering Guangdong Laboratory, Guangzhou, China
- Haitao Ma
- CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Science, Guangzhou, China
- Haitao Ma
- Innovation Academy of South China Sea Ecology and Environmental Engineering (ISEE), Chinese Academy of Sciences, Guangzhou, China
- Haitao Ma
- Southern Marine Science and Engineering Guangdong Laboratory, Guangzhou, China
- Zhiming Xiang
- CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Science, Guangzhou, China
- Zhiming Xiang
- Innovation Academy of South China Sea Ecology and Environmental Engineering (ISEE), Chinese Academy of Sciences, Guangzhou, China
- Zhiming Xiang
- Southern Marine Science and Engineering Guangdong Laboratory, Guangzhou, China
- Jun Li
- CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Science, Guangzhou, China
- Jun Li
- Innovation Academy of South China Sea Ecology and Environmental Engineering (ISEE), Chinese Academy of Sciences, Guangzhou, China
- Jun Li
- Southern Marine Science and Engineering Guangdong Laboratory, Guangzhou, China
- Shu Xiao
- CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Science, Guangzhou, China
- Shu Xiao
- Innovation Academy of South China Sea Ecology and Environmental Engineering (ISEE), Chinese Academy of Sciences, Guangzhou, China
- Shu Xiao
- Southern Marine Science and Engineering Guangdong Laboratory, Guangzhou, China
- Yang Zhang
- CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Science, Guangzhou, China
- Yang Zhang
- Innovation Academy of South China Sea Ecology and Environmental Engineering (ISEE), Chinese Academy of Sciences, Guangzhou, China
- Yang Zhang
- Southern Marine Science and Engineering Guangdong Laboratory, Guangzhou, China
- Ziniu Yu
- CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Science, Guangzhou, China
- Ziniu Yu
- Innovation Academy of South China Sea Ecology and Environmental Engineering (ISEE), Chinese Academy of Sciences, Guangzhou, China
- Ziniu Yu
- Southern Marine Science and Engineering Guangdong Laboratory, Guangzhou, China
- DOI
- https://doi.org/10.3389/fimmu.2020.00416
- Journal volume & issue
-
Vol. 11
Abstract
Invertebrates generally lack adaptive immunity and compensate for this with highly efficient innate immune machineries such as phagocytosis by hemocytes to eradicate invading pathogens. However, how extrinsically cued hemocytes marshal internal signals to accomplish phagocytosis is not yet fully understood. To this end, we established a facile magnetic cell sorting method to enrich professional phagocytes from hemocytes of the Hong Kong oyster (Crassostrea hongkongensis), an ecologically and commercially valuable marine invertebrate. Transcriptomic analysis on presorted cells shows that phagocytes maintain a remarkable array of differentially expressed genes that distinguish them from non-phagocytes, including 352 significantly upregulated genes and 479 downregulated genes. Pathway annotations reveal that focal adhesion and extracellular matrix–receptor interactions were the most conspicuously enriched pathways in phagocytes. Phagocytosis rate dramatically declined in the presence of an FAK inhibitor, confirming importance of the focal adhesion pathway in regulating phagocytosis. In addition, we also found that heparan sulfate proteoglycan (HSPG) families were lineage-specifically expanded in C. hongkongensis and abundantly expressed in phagocytes. Efficiency of phagocytosis and hemocytes aggregation was markedly reduced upon blockage of endogenous synthesis of HSPGs, thus implicating these proteins as key surface receptors in pathogen recognition and initiation of phagocytosis.
Keywords
- phagocytes
- heparan sulfate proteoglycans (HSPGs)
- focal adhesion kinase (FAK)
- transcriptome
- Crassostrea hongkongensis
