Association of Interleukin -17A Genotypes with their Serum Levels in Iraqi Type 2 Diabetic Patients

Abstract

Diabetes mellitus type 2 (T2DM) is one of the most prevalent non-communicable diseases worldwide. Interleukin-17 (IL-17, or IL-17A) is a crucial cytokine that links T cell activation to neutrophil recruitment and activity. In the cytokine storm, up-regulation of T-helper 17 cell cytokine IL-17A, this study aims to investigate the association of polymorphism of IL-17A (rs2275913) gene to their serum levels among Iraqi diabetic type 2 patients. A total of 70 participants, 50 were confirmed previously type 2 diabetic patients, and 20 were apparently healthy. Genetic polymorphism of IL-17AG197A rs (2275913) was carried out using RFLP-PCR. Serum levels of IL-17A were performed using by enzyme-linked immuno-sorbent assay technique (ELISA) using a Human- IL-17A kit. The results of the current study indicated the levels IL-17A were higher in the sera of diabetic patients compared to healthy controls, although there were no significant differences, in patients (243.7233±38.45944), vs. controls (148.8930±11.79280), P= 0.135. . Moreover, T2DM patients with AA and AG genotypes showed higher levels of IL17A than that with GG genotype: 252.5476±50.775 for AA genotype and253.5817±79.13 for AG genotype com-pared to163.5100±8.14105, 115.2900±0.0 for each genotype in controls, P> 0.05.

Keywords

• T2DM, IL-17A, RFLP-PCR, ELISA